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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Transsynaptic tracing to dissect supraspinal serotonergic input regulating the bladder reflex in rats
Neurourology and urodynamics, v 37(8), pp 2487-2494
01 Nov 2018
PMID: 29999191
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
AimsMethodsThis study was designed to determine specific cell groups of the raphe nuclei (RN) that give rise to supraspinal serotonergic projections regulating the bladder reflex.
Anesthetized rats underwent surgery to open the abdomen and expose the bladder. A total of 6 mu L transsynaptic neuronal tracer pseudorabies virus (PRV-152), encoding for green fluorescent protein (GFP), was injected into the bladder detrusor. After 72 or 96h, animals were perfused and the brain was dissected for processing transverse and sagittal sections. Subsequently, fluorescent immunohistochemistry for GFP and Serotonin (5-hydroxytryptamine [5-HT]) was performed in the brain sections. Under the microscope, each RN subset was characterized individually from caudal to rostral according to the atlas. GFP(+) or GFP/5-HT double labeled neurons in each subset were quantified for statistical analysis.
ResultsConclusionsAt 72-h post-infection, very few GFP(+) or GFP/5-HT double-labeled neurons appeared in the brainstem and beyond. In contrast, many labeled neurons were found at these levels after 96h. Quantitative analysis showed that the majority of infected 5-HT+ neurons were located in the pallidus, obscurus, and magnus nuclei. Conversely, very few infected neurons were found in other raphe subsets, that is the pontis, median, dorsal, or linear nuclei. Overall, the raphe magnus had the highest number of GFP-labeled and GFP/5-HT double-labeled cells.
The caudal subsets of RN, especially the raphe magnus, are the main sources of serotonergic input to the lower spinal cord controlling bladder activity.
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Details
- Title
- Transsynaptic tracing to dissect supraspinal serotonergic input regulating the bladder reflex in rats
- Creators
- Jemin Ahn - Drexel UniversityTatiana M. Saltos - Department of Neurobiology and AnatomySpinal Cord Research CenterDrexel University College of MedicinePhiladelphiaPennsylvaniaVeronica J. Tom - Drexel UniversityShaoping Hou - Drexel University
- Publication Details
- Neurourology and urodynamics, v 37(8), pp 2487-2494
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- R01 NS085426; R01 NS099076 / NIH NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01NS099076 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) W81XWH-14-1-0605 / DoD/CDMRP; United States Department of Defense Morton Cure Paralysis Funds
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000448184900023
- Scopus ID
- 2-s2.0-85050941237
- Other Identifier
- 991019167682604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Urology & Nephrology