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Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
Journal of visualized experiments : JoVE, v 2015(100), pp e52825-e52825
29 Jun 2015
PMID: 26167674
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Following injury to the CNS, astrocytes undergo a broad range of biochemical, morphological, and molecular changes collectively referred to as reactive astrogliosis. Reactive astrocytes exert both inflammatory and protective effects that inhibit and promote, respectively, neural repair. The mechanisms underlying the diverse functional properties of reactive astrogliosis are not well understood. Achieving a greater understanding of these mechanisms is critical to developing therapeutic strategies to treat the injured CNS. Here we demonstrate a method to trigger reactive astrogliosis in the adult mouse forebrain using a forebrain stab lesion. This lesion model is simple, reliable, and requires only a stereotaxic device and a scalpel blade to produce the injury. The use of stab lesions as an injury model in the forebrain is well established and amenable to studies addressing a broad range of neuropathological outcomes, such as neuronal degeneration, neuroinflammation, and disruptions in the blood brain barrier (BBB). Thus, the forebrain stab injury model serves as a powerful tool that can be applied for a broad range of studies on the CNS response to trauma.
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Details
- Title
- Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
- Creators
- R Vivian Allahyari - Drexel UniversityA Denise R Garcia - Drexel University
- Publication Details
- Journal of visualized experiments : JoVE, v 2015(100), pp e52825-e52825
- Publisher
- JOVE
- Grant note
- K01 MH097957 / NIMH NIH HHS 5K01MH097957-03 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000361536000044
- Scopus ID
- 2-s2.0-84941243560
- Other Identifier
- 991019168422204721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences