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Journal article
Two-Photon Microperimetry: A Media Opacity-Independent Retinal Function Assay
Translational vision science & technology, v 10(2), pp 11-11
01 Feb 2021
PMID: 34003895
Abstract
Purpose: Compare results obtained using infrared two-photon microperimetry (2PMIR) with conventional visual function tests in healthy subjects of varying ages with and without simulated media opacities. Methods: Subjects from two separate cohort studies completed cone contrast threshold (CCT) testing, conventional microperimetry, visible light microperimetry from a novel device (2PM-Vis), and infrared two-photon microperimetry. The first cohort study, which consisted of six healthy volunteers (23 to 29 years of age), evaluated the effects of simulated media opacities on visual performance testing. Subjects underwent testing on four visual function devices nine separate times under the following conditions: no filter, red filter, green filter, blue filter, light brown filter, dark brown filter, polarized black filter (0 degrees rotation), and polarized black filter (90 degrees rotation). Subjects subsequently performed 2PM-IR and 2PM-Vis testing without a filter in the mydriatic state. The second cohort study evaluated the effect of age on visual test performance in 42 healthy subjects split between two groups (ages 20-40 years and 60-80 years). Results: Retinal sensitivity measured by 2PM-IR demonstrated lower variability than all other devices relying on visible spectrum stimuli. Retinal sensitivity decreased proportionally with the transmittance of light through each filter. CCT scores and retinal sensitivity decreased with age in all testing modalities. Visible spectrum testing modalities demonstrated larger test result differences between young and old patient cohorts; this difference was inversely proportional to the wavelength of the visual function test. Conclusions: 2PM-IR mitigates media opacities that may mask small differences in retinal sensitivity when tested with conventional visual function testing devices. Translational Relevance: Conventional visual function tests that emit visible light may not detect differences in retinal function during the early stages of age-related diseases due to the confounding effects of cataracts. Infrared light, which has greater transmittance through ocular tissue, may reliably quantify retinal sensitivity and thereby detect degenerative changes early on.
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Details
- Title
- Two-Photon Microperimetry: A Media Opacity-Independent Retinal Function Assay
- Creators
- Ang Wei - University of California, IrvineUrmi V. Mehta - College of Osteopathic Medicine of the PacificGrazyna Palczewska - University of California, IrvineAnton M. Palma - Translational SciencesVincent M. Hussey - University of California, IrvineLuke E. Hoffmann - University of California, IrvineAnna Diep - University of California, IrvineKevin Nguyen - Creighton UniversityBryan Le - Drexel UniversitySteven Yone-Shun Chang - University of California, IrvineAndrew W. Browne - University of California, Irvine
- Publication Details
- Translational vision science & technology, v 10(2), pp 11-11
- Publisher
- Assoc Research Vision Ophthalmology Inc
- Number of pages
- 9
- Grant note
- U01 EY025451 / NEI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI) KL2 TR001416 / NCATS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physical Therapy (and Rehabilitation Sciences)
- Web of Science ID
- WOS:000635403100011
- Scopus ID
- 2-s2.0-85103329509
- Other Identifier
- 991019168883404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Ophthalmology