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Two-allergen model reveals complex relationship between IgE crosslinking and degranulation
Journal article   Open access

Two-allergen model reveals complex relationship between IgE crosslinking and degranulation

Michael W Handlogten, Peter E Deak and Basar Bilgicer
Chemistry & biology, v 21(11), pp 1445-1451
20 Nov 2014
PMID: 25308278
url
https://doi.org/10.1016/j.chembiol.2014.08.019View
Published, Version of Record (VoR) Open

Abstract

2,4-Dinitrophenol - chemistry Allergens - chemistry Allergens - immunology Animals Antigen-Antibody Reactions Cattle Cell Degranulation Dansyl Compounds - chemistry Immunoglobulin E - immunology Immunoglobulin E - metabolism Mast Cells - cytology Mast Cells - physiology Models, Biological Rats Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - immunology
Allergy is an immune response to complex mixtures of multiple allergens, yet current models use a single synthetic allergen. Multiple allergens were modeled using two well-defined tetravalent allergens, each specific for a distinct IgE, thus enabling a systematic approach to evaluate the effect of each allergen and percentage of allergen-specific IgE on mast cell degranulation. We found the overall degranulation response caused by two allergens is additive for low allergen concentrations or low percent specific IgE, does not change for moderate allergen concentrations with moderate to high percent specific IgE, and is reduced for high allergen concentrations with moderate to high percent specific IgE. These results provide further evidence that supraoptimal IgE crosslinking decreases the degranulation response and establishes the two-allergen model as a relevant experimental system to elucidate mast cell degranulation mechanisms.

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Biochemistry & Molecular Biology
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