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Journal article
Type V collagen exhibits distinct regulatory activities in TMJ articular disc versus condylar cartilage during postnatal growth and remodeling
Acta biomaterialia, v 189, pp 192-207
17 Nov 2024
PMID: 39362448
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Abstract
Understanding matrix molecular activities that regulate the postnatal growth and remodeling of temporomandibular joint (TMJ) condylar cartilage and articular disc will enable the development of effective regenerative strategies targeting TMJ disorder. This study elucidated the distinct roles of type V collagen (collagen V) in regulating these two units. Studying the TMJ of young adult Col5a1+/− mice, we found loss of collagen V resulted in substantial changes in the proliferation, clustering, and density of progenitors in condylar cartilage, but did not have a major impact on disc cells that are more fibroblast-like. Although loss of collagen V led to thickened collagen fibrils with increased heterogeneity in the disc, there were no significant changes in local micromodulus except for a reduction at the posterior end of the inferior side. Following the induction of aberrant occlusal loading by the unilateral anterior crossbite (UAC) procedure, both wild-type (WT) and Col5a1+/− condylar cartilage exhibited salient remodeling, and Col5a1+/− condyle developed more pronounced degeneration and hypertrophy at the posterior end than the WT. In contrast, neither UAC nor collagen V deficiency induced marked changes in the morphology or mechanical properties of the disc. Together, our findings highlight the distinct roles of collagen V in regulating these two units during postnatal growth and remodeling, emphasizing its more crucial role in condylar cartilage due to its impact on the highly mechanosensitive progenitors. Results thus provide the foundation for using collagen V to improve the regeneration of TMJ and the care of patients with TMJ disorder.
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Details
- Title
- Type V collagen exhibits distinct regulatory activities in TMJ articular disc versus condylar cartilage during postnatal growth and remodeling
- Creators
- Prashant Chandrasekaran - Drexel UniversityAbdulaziz Alanazi - Drexel UniversityBryan Kwok - Drexel University, School of Biomedical Engineering, Science, and Health SystemsQing Li - Drexel UniversityGirish Viraraghavan - Drexel UniversitySriram Balasubramanian - Drexel University, School of Biomedical Engineering, Science, and Health SystemsDavid B. FrankX. Lucas Lu - University of DelawareDavid E. Birk - University of South FloridaRobert L. Mauck - University of PennsylvaniaNathaniel A. Dyment - University of PennsylvaniaEiki Koyama - Children's Hospital of PhiladelphiaLin Han (Corresponding Author) - Drexel University, School of Biomedical Engineering, Science, and Health Systems
- Publication Details
- Acta biomaterialia, v 189, pp 192-207
- Publisher
- Elsevier
- Number of pages
- 16
- Grants
- Grant note
- This work was financially supported by the National Science Foundation (NSF) Grant CMMI-1751898 (to LH), National Institutes of Health (NIH) Grant R21DE029567 (to LH), as well as NIH Grant P30AR069619 to the Penn Center for Musculoskeletal Disorders. We thank Dr. L. J. Soslowsky (University of Pennsylvania) for the kind help with murine TMJ samples.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001360197200001
- Scopus ID
- 2-s2.0-85206892336
- Other Identifier
- 991021903506004721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials