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Journal article
Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin
Proceedings of the National Academy of Sciences - PNAS, v 104(39), pp 15508-15513
25 Sep 2007
PMCID: PMC2000545
PMID: 17873062
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Genes encoding type VI secretion systems (T6SS) are widely distributed in pathogenic Gram-negative bacterial species. In
Vibrio cholerae
, T6SS have been found to secrete three related proteins extracellularly, VgrG-1, VgrG-2, and VgrG-3. VgrG-1 can covalently cross-link actin
in vitro
, and this activity was used to demonstrate that
V. cholerae
can translocate VgrG-1 into macrophages by a T6SS-dependent mechanism. Protein structure search algorithms predict that VgrG-related proteins likely assemble into a trimeric complex that is analogous to that formed by the two trimeric proteins gp27 and gp5 that make up the baseplate “tail spike” of
Escherichia coli
bacteriophage T4. VgrG-1 was shown to interact with itself, VgrG-2, and VgrG-3, suggesting that such a complex does form. Because the phage tail spike protein complex acts as a membrane-penetrating structure as well as a conduit for the passage of DNA into phage-infected cells, we propose that the VgrG components of the T6SS apparatus may assemble a “cell-puncturing device” analogous to phage tail spikes to deliver effector protein domains through membranes of target host cells.
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Details
- Title
- Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin
- Creators
- Stefan Pukatzki - Harvard UniversityAmy T. Ma - Harvard Medical SchoolAndrew T. Revel - Harvard UniversityDerek Sturtevant - Harvard UniversityJohn J. Mekalanos - Harvard University
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 104(39), pp 15508-15513
- Publisher
- National Academy of Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000249806900060
- Scopus ID
- 2-s2.0-34848882221
- Other Identifier
- 991020830153504721
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- Web of Science research areas
- Biochemistry & Molecular Biology