Journal article
USP11 Promotes Endothelial Apoptosis-Resistance in Pulmonary Arterial Hypertension by Deubiquitinating HINT3
Journal of respiratory biology and translational medicine, v 2(1), 10002
01 Mar 2025
PMID: 40376595
Abstract
Pulmonary arterial hypertension (PAH) is a progressive, lethal, and incurable disease of the pulmonary vasculature. A previous genome-wide association study (GWAS) with Affymetrix microarray analysis data exhibited elevated histidine triad nucleotide-binding protein 3 (HINT3) in the lung samples of PAH compared to control subjects (failed donors, FD) and the positive correlations of HINT3 with deubiquitinase USP11 and B-cell lymphoma 2 (BCL2). In this study, we aim to investigate the roles and interplay of USP11 and HINT3 in the apoptosis resistance of PAH. The levels of USP11 and HINT3 were increased in the lungs of idiopathic PAH (IPAH) patients and Hypoxia/Sugen-treated mice. USP11 and HINT3 interacted physically, as shown by co-immunoprecipitation (co-IP) assay in human pulmonary arterial endothelial cells (HPAECs). HINT3 was degraded by polyubiquitination, which was reversed by USP11. Furthermore, HINT3 interacted with the anti-apoptotic mediator, BCL2. Overexpression of USP11 increased BCL2 content, congruent to elevated lung tissue levels seen in IPAH patients and Hypoxia/Sugen-treated mice. Conversely, the knockdown of HINT3 function led to a depletion of BCL2. Thus, we conclude that USP11 stabilizes HINT3 activation, which contributes to endothelial apoptosis-resistance of pulmonary arterial endothelial cells in PAH. This can potentially be a novel therapeutic target for ubiquitination modulators for PAH.
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Details
- Title
- USP11 Promotes Endothelial Apoptosis-Resistance in Pulmonary Arterial Hypertension by Deubiquitinating HINT3
- Creators
- Bum-Yong KangJiwoong Choi - University of KansasVictor Tseng - Deep Space Industries (United States)Yutong Zhao - The Ohio State UniversityJing ZhaoRobert S. Stearman - Indiana University – Purdue University IndianapolisWilbur A. Lam - Georgia Institute of TechnologyViranuj Sueblinvong - Emory UniversityBenjamin T. Kopp - Cystic Fibrosis FoundationMichael J. Passineau - Allegheny Health NetworkChangwon Park - Louisiana State University Health Sciences Center ShreveportJohn Lister - Allegheny Health NetworkRaymond J. Benza - Mount Sinai HospitalAndrew J. Jang (Corresponding Author) - Drexel University
- Publication Details
- Journal of respiratory biology and translational medicine, v 2(1), 10002
- Publisher
- SCIE Publishing
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- General Internal Medicine
- Scopus ID
- 2-s2.0-105010396087
- Other Identifier
- 991022197334704721