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Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips
Journal article   Open access   Peer reviewed

Ultra-Fast Low Concentration Detection of Candida Pathogens Utilizing High Resolution Micropore Chips

Rafael Mulero, Dong Heun Lee, Michele A Kutzler, Jeffrey M Jacobson and Min Jun Kim
Sensors (Basel, Switzerland), v 9(3), pp 1590-1598
Mar 2009
PMID: 22573974
url
https://doi.org/10.3390/s90301590View
Published, Version of Record (VoR) Open

Abstract

ionic current blockage low concentration detection pathogens Candida micropore Communication
Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods’ complexity for both identification and quantification capabilities in mixed blood samples.

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4 citations in Scopus

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#3 Good Health and Well-Being

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Web of Science research areas
Chemistry, Analytical
Engineering, Electrical & Electronic
Instruments & Instrumentation
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