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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Ultrastructural relationship between the mu opioid receptor and its interacting protein, GPR177, in striatal neurons
Brain research, v 1358, pp 71-80
28 Oct 2010
PMID: 20813097
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
GPR177, the mammalian ortholog of Drosophila Wntless/Evi/Sprinter, was recently identified as a novel mu-opioid receptor (MOR) interacting protein. GPR177 is a transmembrane protein pivotal to mediating the secretion of Wnt signaling proteins. Wnt proteins, in turn, are essential in regulating neuronal development, a phenomenon inhibited upon chronic exposure to MOR agonists such as morphine and heroin. We previously showed that GPR177 and MOR are co-localized in the mouse dorsolateral striatum; however, the nature of this interaction was not fully elucidated. Therefore, in the present study, we examined cellular substrates for interactions between GPR177 and MOR using a combined immunogold-silver and peroxidase detection approach in coronal sections in the dorsolateral segment of the striatum. Semi-quantitative analysis of the ultrastructural distribution of GPR177 and MOR in striatal somata and in dendritic processes showed that, of the somata and dendritic processes exhibiting GPR177, 32% contained MOR immunolabeling while for profiles exhibiting MOR, 37% also contained GPR177 immunoreactivity. GPR177-labeled particles were localized predominantly along both the plasma membrane and within the cytoplasm of MOR-labeled dendrites. Somata and dendritic processes that contained both GPR177 and MOR more often received symmetric (inhibitory-type) synapses from unlabeled axon terminals. To further define the phenotype of GPR177 and MOR-containing cellular profiles, triple immunofluorescence detection showed that GPR177 and MOR are localized in neurons containing the opioid peptide, enkephalin, within the dorsolateral striatum. The results provide an anatomical substrate for interactions between MOR and its interacting protein, GPR177, in striatal opioid-containing neurons that may underlie the morphological alterations produced in neurons by chronic opiate use. (C) 2010 Elsevier B.V. All rights reserved.
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Details
- Title
- Ultrastructural relationship between the mu opioid receptor and its interacting protein, GPR177, in striatal neurons
- Creators
- Arith-Ruth S. Reyes - Jefferson Hospital for NeuroscienceRobert Levenson - Penn State Milton S. Hershey Medical CenterWade Berrettini - University of PennsylvaniaElisabeth J. Van Bockstaele - Thomas Jefferson University
- Publication Details
- Brain research, v 1358, pp 71-80
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- R01DA009082 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission P20 DA 025995; DA 09082 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000283814800009
- Scopus ID
- 2-s2.0-77957372856
- Other Identifier
- 991021903414204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences