Journal article
Unexpected nuclear localization of a chimeric beta-galactosidase lacZ reporter gene product in mammalian cells
Biochemistry and molecular biology international, v 36(3), pp 483-490
01 Jul 1995
PMID: 7549945
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A lacZ cassette was designed to include a synthetic amino terminus optimized for translation in eukaryotic cells, as well as multiple restriction sites for the insertion of heterologous regulatory elements both 5' and 3' of the reporter. The cassette was placed under the control of the metallothionein promoter in combination with the SV40 enhancer and this plasmid was introduced into mammalian cells. High levels of beta-galactosidase were observed in several cell types, demonstrating efficient expression of the reporter. Unexpectedly, most of the chromogenic reaction product appeared to be intra- or peri-nuclear, indicating that the enzyme is similarly localized. The synthetic amino terminus does not resemble known nuclear localization signals and thus may constitute a novel signal.
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Details
- Title
- Unexpected nuclear localization of a chimeric beta-galactosidase lacZ reporter gene product in mammalian cells
- Creators
- R McInnis - Roger Williams Medical CenterR A PerkinsonB A KuoP A Norton
- Publication Details
- Biochemistry and molecular biology international, v 36(3), pp 483-490
- Grant note
- GM46402 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1995UG01700003
- Scopus ID
- 2-s2.0-0029551126
- Other Identifier
- 991020830292804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology