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UniAlign: protein structure alignment meets evolution
Journal article   Open access   Peer reviewed

UniAlign: protein structure alignment meets evolution

Chunyu Zhao and Ahmet Sacan
Bioinformatics (Oxford, England), v 31(19), pp 3139-3146
01 Oct 2015
PMID: 26059715
url
https://doi.org/10.1093/bioinformatics/btv354View
Published, Version of Record (VoR) Open

Abstract

Sequence Homology, Amino Acid Amino Acid Sequence Sequence Alignment - methods Databases, Protein Humans Conserved Sequence Molecular Sequence Data Software Structural Homology, Protein Proteins - chemistry Evolution, Molecular
During the evolution, functional sites on the surface of the protein as well as the hydrophobic core maintaining the structural integrity are well-conserved. However, available protein structure alignment methods align protein structures based solely on the 3D geometric similarity, limiting their ability to detect functionally relevant correspondences between the residues of the proteins, especially for distantly related homologous proteins. In this article, we propose a new protein pairwise structure alignment algorithm (UniAlign) that incorporates additional evolutionary information captured in the form of sequence similarity, sequence profiles and residue conservation. We define a per-residue score (UniScore) as a weighted sum of these and other features and develop an iterative optimization procedure to search for an alignment with the best overall UniScore. Our extensive experiments on CDD, HOMSTRAD and BAliBASE benchmark datasets show that UniAlign outperforms commonly used structure alignment methods. We further demonstrate UniAlign's ability to develop family-specific models to drastically improve the quality of the alignments. UniAlign is available as a web service at: http://sacan.biomed.drexel.edu/unialign ahmet.sacan@drexel.edu Supplementary data are available at Bioinformatics online.

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Web of Science research areas
Biochemical Research Methods
Biotechnology & Applied Microbiology
Computer Science, Interdisciplinary Applications
Mathematical & Computational Biology
Statistics & Probability
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