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Journal article
Unique cellular network formation guided by heterostructures based on reduced graphene oxide - Ti3C2Tx MXene hydrogels
Acta biomaterialia, v 115
01 Oct 2020
PMID: 32795646
Abstract
Two-dimensional (2D) materials remain highly interesting for assembling three-dimensional (3D) structures, amongst others, in the form of macroscopic hydrogels. Herein, we present a novel approach for inducing chemical inter-sheet crosslinks via an ethylenediamine mediated reaction between Ti3C2Tx and graphene oxide in order to obtain a reduced graphene oxide-MXene (rGO-MXene) hydrogel. The composite hydrogels are hydrophilic with a stiffness of similar to 20 kPa. They also possess a unique inter-connected porous architecture, which led to a hitherto unprecedented ability of human cells across three different types, epithelial adenocarcinoma, neuroblastoma and fibroblasts, to form inter-connected three-dimensional networks. The attachments of the cells to the rGO-MXene hydrogels were superior to those of the sole rGO-control gels. This phenomenon stems from the strong affinity of cellular protrusions (neurites, lamellipodia and filopodia) to grow and connect along architectural network paths within the rGO-MXene hydrogel, which could lead to advanced control over macroscopic formations of cellular networks for technologically relevant bioengineering applications, including tissue engineering and personalized diagnostic networks-on-chip.
Statement of Significance
Conventional hydrogels are made of interconnected polymeric fibres. Unlike conventional case, we used hydrothermal and chemical approach to form interconnected porous hydrogels made of two-dimensional flakes from graphene oxide and metal carbide from a new family of MXenes (Ti3C2Tx). This way, we formed three-dimensional porous hydrogels with unique porous architecture of well-suited chemical surfaces and stiffness. Cells from three different types cultured on these scaffolds formed extended three-dimensional networks - a feature of extended cellular proliferation and pre-requisite for formation of organoids. Considering the studied 2D materials typically constitute materials exhibiting enhanced super-capacitor performances, our study points towards better understanding of design of tissue engineering materials for the future bioengineering fields including personalized diagnostic networks-on-chip, such as artificial heart actuators. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd.
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Details
- Title
- Unique cellular network formation guided by heterostructures based on reduced graphene oxide - Ti3C2Tx MXene hydrogels
- Creators
- Jacek K. Wychowaniec - Adam Mickiewicz University in PoznańJagoda Litowczenko - Adam Mickiewicz University in PoznańKrzysztof Tadyszak - Adam Mickiewicz University in PoznańVarun Natu - Drexel UniversityClaudia Aparicio - Regional Centre of Advanced Technologies and MaterialsBarbara Peplinska - Adam Mickiewicz University in PoznańMichel W. Barsoum - Drexel UniversityMichal Otyepka - Regional Centre of Advanced Technologies and MaterialsBlazej Scheibe - Adam Mickiewicz Univ Pozna, NanoBioMed Ctr, Wszechnicy Piastowskiej 3, PL-61614 Poznan, Poland
- Publication Details
- Acta biomaterialia, v 115
- Publisher
- Elsevier
- Number of pages
- 12
- Grant note
- CA15107 / COST Action; European Cooperation in Science and Technology (COST) PRELUDIUM 2016/23/N/ST5/00955; SONATA 2016/21/D/ST3/00975; SONATA 2014/13/D/ST5/02824 / National Science Centre (NSC) 683024 / ERC-CoG programme CZ.02.1.01/0.0/0.0/16_019/0000754 / Ministry of Education, Youths and Sports of the Czech Republic; Ministry of Education, Youth & Sports - Czech Republic COST Association
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000577511200007
- Scopus ID
- 2-s2.0-85089827845
- Other Identifier
- 991019169009604721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials