Journal article
Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma
Tumor biology, v 37(6), pp 8305-8315
Jun 2016
PMID: 26729195
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
LIM and SH3 protein 1 (LASP-1) is demonstrated to play a key role in occurrence and development of tumors. However, the expression and function of LASP-1 in cholangiocarcinoma (CCA) remain largely unexplored. This study aimed to investigate the effect of regulated LASP-1 expression on migration, invasion, proliferation, and apoptosis of CCA cells and on tumorigenesis in vivo, and to examine clinico-oncological correlates of LASP-1 expression. Expression of LASP-1 by immunohistochemistry was evaluated in CCA tissue samples. HCCC-9810 and RBE cells were transfected with the LASP-1 small interfering RNA (siRNA), and the effect of knocking down LASP-1 gene expression on cell migration, invasion, proliferation, and apoptosis were examined by wound healing, transwell assays, CCK-8 assays, colony formation, and flow cytometry assays, respectively. Xenograft tumor model was used to validate the effect of downregulated LASP-1 in vivo. Our results demonstrated that LASP-1 was over-expressed in CCA tissues, positively correlating with larger tumors, poor histological differentiation, lymph node metastasis, advanced TNM stage, and poor prognosis in CCA patients (P < 0.05). Downregulation of LASP-1 in HCCC-9810 and RBE cell lines significantly increased cell apoptosis and suppressed cell migration, invasion, and proliferation in vitro and tumorigenesis in vivo. Our results indicate that LASP-1 may essentially involve in the metastasis and growth of CCA and clinical significance of LASP-1 may reside in function as a biomarker to predict prognosis and as a promising therapeutic strategy for CCA patients by the inhibition of LASP-1 expression.
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Details
- Title
- Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma
- Creators
- Hongchen Zhang - Shanghai Jiao Tong UniversityZhizhen Li - Shanghai Jiao Tong UniversityBingfeng Chu - Shanghai Jiao Tong UniversityFei Zhang - Shanghai Jiao Tong UniversityYijian Zhang - Shanghai Jiao Tong UniversityFayong Ke - Shanghai Jiao Tong UniversityYuanyuan Chen - Shanghai Jiao Tong UniversityYi Xu - Shanghai Jiao Tong UniversityShibo Liu - Shanghai Jiao Tong UniversityShuai Zhao - Shanghai Jiao Tong UniversityHaibin Liang - Shanghai Jiao Tong UniversityMingzhe Weng - Shanghai Jiao Tong UniversityXiangsong Wu - Shanghai Jiao Tong UniversityMaolan Li - Shanghai Jiao Tong UniversityWenguang Wu - Shanghai Jiao Tong UniversityZhiwei Quan - Shanghai Jiao Tong UniversityYingbin Liu - Shanghai Jiao Tong UniversityYong Zhang - Shanghai Jiao Tong UniversityWei Gong - Shanghai Jiao Tong UniversityHualou Liang - School of Biomedical Engineering, Science, and Health Systems (1997-)
- Publication Details
- Tumor biology, v 37(6), pp 8305-8315
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000376464700130
- Scopus ID
- 2-s2.0-84952916431
- Other Identifier
- 991019320513304721
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- Oncology