Journal article
Upregulation of Functional Ryanodine Receptors duringin VitroAging of Human Diploid Fibroblasts
Biochemical and biophysical research communications, v 245(1), pp 50-52
07 Apr 1998
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We demonstrate for the first time that cellular agingin vitrois accompanied by a dramatic elevation in the levels of ryanodine receptor-bearing Ca2+channels. These channels normally reside within microsomal membranes and gate Ca2+release from intracellular stores. We therefore measured cytosolic Ca2+levels in ‘young’ (30 mean population doublings, MPDs) and ‘senescent’ (53 to 58 MPDs) human diploid fibroblasts (HDFs). Application of the known ryanodine receptor modulators, caffeine or cyclic adenosine diphosphate-ribose (cADPr), triggered cytosolic Ca2+signals in both young and senescent cells. The signal magnitude however was significantly greater in senescent compared with young HDFs. In parallel, incubation with a highly specific anti-ryanodine receptor antiserum resulted in specific immunofluorescence only in senescent HDFs. We envisage that elevated levels of functional ryanodine receptors may underlie the defective Ca2+handling and cellular degeneration that occurs with aging.
Metrics
Details
- Title
- Upregulation of Functional Ryanodine Receptors duringin VitroAging of Human Diploid Fibroblasts
- Creators
- Ming-Shang Huang - University of Arkansas for Medical SciencesOlugbenga Adebanjo - Allegheny University of the Health SciencesBaljit S. Moonga - Allegheny University of the Health SciencesSamuel Goldstein - University of Arkansas for Medical SciencesF.Anthony Lai - Allegheny University of the Health SciencesDavid A. Lipschitz - Allegheny University of the Health SciencesMone Zaidi - Allegheny University of the Health Sciences
- Publication Details
- Biochemical and biophysical research communications, v 245(1), pp 50-52
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Finance
- Web of Science ID
- WOS:000073279400009
- Scopus ID
- 2-s2.0-0032492552
- Other Identifier
- 991019168114404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
Source: SDGs in the Output
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics