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Journal article
Upregulation of Glycans Containing 3’ Fucose in a Subset of Pancreatic Cancers Uncovered Using Fusion-Tagged Lectins
Journal of proteome research, v 14(6), pp 2594-2605
12 May 2015
PMID: 25938165
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The fucose post-translational modification is frequently increased in pancreatic cancer, thus forming the basis for promising biomarkers, but a subset of pancreatic cancer patients does not elevate the known fucose-containing biomarkers. We hypothesized that such patients elevate glycan motifs with fucose in linkages and contexts different from the known fucose-containing biomarkers. We used a database of glycan array data to identify the lectins CCL2 to detect glycan motifs with fucose in a 3’ linkage; CGL2 for motifs with fucose in a 2’ linkage; and RSL for fucose in all linkages. We used several practical methods to test the lectins and determine the optimal mode of detection, and we then tested whether the lectins detected glycans in pancreatic cancer patients who did not elevate the sialyl-Lewis A glycan, which is upregulated in ~75% of pancreatic adenocarcinomas. Patients who did not upregulate sialyl-Lewis A, which contains fucose in a 4’ linkage, tended to upregulate fucose in a 3’ linkage, as detected by CCL2, but they did not upregulate total fucose or fucose in a 2’ linkage. CCL2 binding was high in cancerous epithelia from pancreatic tumors, including areas negative for sialyl-Lewis A and a related motif containing 3’ fucose, sialyl-Lewis X. Thus glycans containing 3’ fucose may complement sialyl-Lewis A to contribute to improved detection of pancreatic cancer. Furthermore, the use of panels of recombinant lectins may uncover details about glycosylation that could be important for characterizing and detecting cancer.
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Details
- Title
- Upregulation of Glycans Containing 3’ Fucose in a Subset of Pancreatic Cancers Uncovered Using Fusion-Tagged Lectins
- Creators
- Sudhir Singh - Van Andel InstituteKuntal Pal - Van Andel InstituteJessica Yadav - Van Andel InstituteHuiyuan Tang - Van Andel InstituteKatie Partyka - Van Andel InstituteDoron Kletter - Palo Alto Research CenterPeter Hsueh - Van Andel InstituteElliot Ensink - Van Andel InstituteK C Birendra - Spectrum HealthGalen Hostetter - Van Andel InstituteH. Eric Xu - Van Andel InstituteMarshall Bern - Palo Alto Research CenterDavid F. Smith - Emory UniversityAnand S. Mehta - Drexel UniversityRandall Brand - University of Pittsburgh Medical CenterKarsten Melcher - Van Andel InstituteBrian B. Haab - Van Andel InstituteSabina B Singh - Pediatrics
- Publication Details
- Journal of proteome research, v 14(6), pp 2594-2605
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000355962000021
- Scopus ID
- 2-s2.0-84930608347
- Other Identifier
- 991019174016904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods