Journal article
Upregulation of adrenergic beta receptor subtypes in the senescent rat heart
Mechanisms of ageing and development, v 49(3), pp 235-243
1989
PMID: 2554074
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Beta receptors in older hearts respond to procedures which cause upregulation (N. Turner
et al., J. Gerontol., 1989, in press). To determine whether a particular beta receptor subtype is responsible for the development of upregulation as a function of age, we studied the radio of beta
1- to beta
2-adrenergic receptors in the membrane preparations from the ventricles of Fischer-344 hearts at 6 and 24 months of age. The animals were injected with 6-hydroxydopamine hydrobomide (6-OHDA) (2 × 50 mg/kg, i.v.) on days 1 and 8 and they were decapitated on day 15. The depletion of norepinephrine in the heart was about 86% in each age group. [
125I]iodopindolol (IPIN) was used as the radioligand at the final concentration of 110 pM. Inhibition of specific IPIN binding was studied by adding ICI 89,406 (beta
1-selective antagnist) and ICI 118,551 (beta
2-selective antagonist) at 25 pM to 40μM. The relative proportions of the beta receptor subtypes were determined using a competition radioligand selective binding and computer modeling technique. The ventricles contained about 67% beta
1 and 33% beta
2-adrenergic receptors in hearts isolated from 6- and 24-month old rats; the ratio remained the same in sympathectomized animals. These data suggest that both subtypes of cardiac adrenergic receptors participate in the response to chemical denervation of 6-OHDA regardless of age.
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Details
- Title
- Upregulation of adrenergic beta receptor subtypes in the senescent rat heart
- Creators
- Nihal Tumer - Drexel UniversityWayne T. Houck - Drexel UniversityJay Roberts - Drexel UniversityJames R Roberts - Emergency Medicine
- Publication Details
- Mechanisms of ageing and development, v 49(3), pp 235-243
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Emergency Medicine
- Web of Science ID
- WOS:A1989AN98900004
- Scopus ID
- 2-s2.0-0024365483
- Other Identifier
- 991019184067404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Geriatrics & Gerontology