Journal article
Upstream Stimulatory Factor Family Binds to the Herpes Simplex Virus Type 1 Latency-Associated Transcript Promoter
Virology (New York, N.Y.), v 230(2), pp 381-391
14 Apr 1997
PMID: 9143295
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) promoter 1 (LP1) is the only viral promoter that exhibits detectable transcriptional activity during a latent HSV infection. The LAT promoter-binding factor (LPBF) regulatory sequence (nucleotides −65 to −72 relative to the transcriptional start site of the 8.3-kb primary transcript) closely resembles the core recognition sequence required for binding members of the upstream stimulatory factor (USF)/major late transcription factor (MLTF) family. In this analysis, we demonstrate that oligonucleotides containing either the LPBF recognition sequence or the USF/MLTF recognition sequences from previously described promoters bind cellular factors which exhibit very similar mobilities in electrophoretic mobility shift (EMS) analyses. We also observe a high degree of similarity in competition profiles obtained in competition EMS analyses utilizing oligonucleotides containing recognition sequences for either LPBF or USF/MLTF. Furthermore, antibody supershift EMS analyses have demonstrated that the factors binding the LPBF or USF/MLTF recognition sites in these oligonucleotides are antigenically related, if not identical, and that greater than 90% of the LPBF-binding activity is antigenically related to USF. In addition, we demonstrate that both forms ofin vitro-translated USF proteins (43 and 44 kDa) bind to the LPBF recognition sequence within HSV-1 LP1. Taken together, these data indicate that USF is capable of binding to the HSV-1 LPBF recognition sequence and that USF is a major LPBF-binding activity in cells of neuronal and nonneuronal lineage. These data further support the hypothesis that USF may indeed play a significant role in the transcriptional activity of HSV-1 LP1.
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Details
- Title
- Upstream Stimulatory Factor Family Binds to the Herpes Simplex Virus Type 1 Latency-Associated Transcript Promoter
- Creators
- Joseph J KennyScott MillhouseMichael WotringBrian Wigdahl
- Publication Details
- Virology (New York, N.Y.), v 230(2), pp 381-391
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1997WV20400026
- Scopus ID
- 2-s2.0-0030916284
- Other Identifier
- 991014877802404721
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