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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v 2021(171)
01 May 2021
PMID: 34096919
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Small extracellular vesicles (sEVs) are 50-150 nm vesicles secreted by all cells and present in bodily fluids. sEVs transfer biomolecules such as RNA, proteins, and lipids from donor to acceptor cells, making them key signaling mediators between cells. In the central nervous system (CNS), sEVs can mediate intercellular signaling, including neuroimmune interactions. sEV functions can be studied by tracking the uptake of labeled sEVs in recipient cells both in vitro and in vivo. This paper describes the labeling of sEVs from the conditioned media of RAW 264.7 macrophage cells using a PKH membrane dye. It shows the uptake of different concentrations of labeled sEVs at multiple time points by Neuro-2a cells and primary astrocytes in vitro. Also shown is the uptake of sEVs delivered intrathecally in mouse spinal cord neurons, astrocytes, and microglia visualized by confocal microscopy. The representative results demonstrate time-dependent variation in the uptake of sEVs by different cells, which can help confirm successful sEVs delivery into the spinal cord.
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Details
- Title
- Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
- Creators
- Richa Gupta - Drexel UniversityXuan Luo - Drexel UniversityZhucheng Lin - Drexel UniversityYuzhen Tian - Drexel UniversitySeena K. Ajit - Drexel University
- Publication Details
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v 2021(171)
- Publisher
- Journal Of Visualized Experiments
- Number of pages
- 17
- Grant note
- Pennsylvania Department of Health Commonwealth Universal Research Enhancement (CURE) R01NS102836 / NIH NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000658340400080
- Scopus ID
- 2-s2.0-85107641998
- Other Identifier
- 991019168012904721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology