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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research
Clinical biochemistry, v 39(11), pp 1071-1079
2006
PMID: 17010960
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We developed assays for measurement of urinary βLH and βFSH under collection and storage conditions typical of non-clinical research settings.
IEMAs for free βLH and total βFSH were validated by standard methods. Stability of urinary βLH and βFSH was tested across freeze–thaws and stored long term at 4°C or −
20°C, or short term at room temperature, and with heating to dissociate the subunits.
The IEMAs exhibited acceptable parallelism, specificity, recovery (averaging 100% for βLH, 97% for βFSH), imprecision (maximum within-run and between run CVs, respectively, 4.8% and 25.7% for βLH, 5.6% and 17.0% for βFSH), and minimum detectable dose (2.5 pmol/L for βLH, 6.8 pmol/L for βFSH). Urine and serum measures were highly correlated (
r
=
0.95 for LH, 0.86 for FSH). There was no consistent decline with any storage type. Dissociation of subunits by heating was needed for βLH, but not βFSH.
These IEMAs measure free βLH and total βFSH, overcoming inter-individual variability in, and collection and storage effects on, subunit dissociation, without the need for urine preservatives.
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Details
- Title
- Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research
- Creators
- Eleanor Brindle - University of WashingtonRebecca C. Miller - Drexel UniversityJane B. Shofer - University of WashingtonNancy A. Klein - Seattle Reproductive MedicineMichael R. Soules - Seattle Reproductive MedicineKathleen A. O'Connor - University of WashingtonWilliam Clement Regli
- Publication Details
- Clinical biochemistry, v 39(11), pp 1071-1079
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000242334000005
- Scopus ID
- 2-s2.0-33750951468
- Other Identifier
- 991019357639904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medical Laboratory Technology