Journal article
Urinary glycan markers for disease
Biochemical Society transactions, v 39(1), pp 393-398
01 Feb 2011
PMID: 21265811
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Robust assays for the isolation and characterization of urinary FOS (free oligosaccharides) have been developed to screen patients for altered protein and/or lipid glycosylation. A FOS analysis can therefore identify potential biomarkers for hepatocellular carcinoma, since variations in glycosylation as a result of tumorigenecity should be detectable in the FOS of patients. HCC (hepatocellular carcinoma) accounts for 80-90% of all liver cancers. It occurs more often in men than women and occurs mostly in people 50-60 years old. The disease is more common in parts of Africa and Asia than in North or South America and Europe. Using a combination of solid-phase extraction techniques and affinity chromatography, followed by separation of urinary FOS by NP (normal phase)-HPLC and HIAX (hydrophilic interaction and anion-exchange)-HPLC, more than 200 different species have been identified in patient samples. The high incidence of small sialylated oligosaccharides in HCC patients suggests that pro-inflammatory markers may be detected as early indicators of disease progression. In addition, the methods developed here to isolate and analyse excreted glycoprotein- and glycosphingolipid-bound oligosaccharides have been used to characterize changes in metabolic processes that underlie a number of human genetic disorders. The ability to predict disease status in microlitre amounts of readily available non-invasive urine samples indicates that rapid methods for screening can be developed.
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Details
- Title
- Urinary glycan markers for disease
- Creators
- Dominic S. Alonzi - University of OxfordYing-Hsiu Su - Drexel UniversityTerry D. Butters - University of Oxford
- Publication Details
- Biochemical Society transactions, v 39(1), pp 393-398
- Publisher
- Portland Press Ltd
- Number of pages
- 6
- Grant note
- R01CA125642 / National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Oxford Glycobiology Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000287438000073
- Scopus ID
- 2-s2.0-79551488998
- Other Identifier
- 991019167611504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology