Journal article
Use of NAD(P)H-fluorescence for monitoring the response of starved cells of Catharanthus roseus in suspension to metabolic perturbations
Journal of biotechnology, v 23(1), pp 83-93
1992
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this investigation we have measured, on-line, the concentration of NAD(P)H of a quasi-steady state culture of
Catharanthus roseus subjected to a variety of different metabolic perturbations, including transition from aerobic to anaerobic conditions, and glucose pulse feeding. The NAD(P)H fluorescence was found to qualitatively represent the metabolic state of cells during substrate perturbation. NAD(P)H fluorescence increased immediately upon aerobic-anaerobic transition and decreased immediately after returning to aerobic conditions. NAD(P)H fluorescence response following a decrease or increase in dissolved oxygen tension indicates that the cells are in an aerobic (or oxidized) state at as low an oxygen tension as 11% air saturation.
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Details
- Title
- Use of NAD(P)H-fluorescence for monitoring the response of starved cells of Catharanthus roseus in suspension to metabolic perturbations
- Creators
- E.C. Asali - Lehigh UniversityR. Mutharasan - Drexel UniversityA.E. Humphrey - Lehigh University
- Publication Details
- Journal of biotechnology, v 23(1), pp 83-93
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:A1992HK42000006
- Scopus ID
- 2-s2.0-0026573855
- Other Identifier
- 991019173425204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology