Journal article
Use of dual-labeled microcapsules to discern the physiological fates of assimilated carbohydrate, protein carbon, and protein nitrogen in suspension-feeding organisms
Limnology and oceanography, v 41(2), pp 208-215
01 Mar 1996
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A mixture of either N-15-labeled protein and C-14-labeled carbohydrate (type 1) or dual-labeled C-14-N-15 protein and unlabeled carbohydrate (type 2) was microencapsulated and fed to mussels (Mytilus edulis). Defecation, excretion, respiration, and incorporation of both isotopes were quantified to compare the relative utilization of ingested carbohydrate (C-14 in type 1), protein C (C-14 in type 2), and protein N (N-15 in types 1 and 2). Assimilation efficiencies were in the order protein N (26.6%) > carbohydrate (16.3%) > protein C (8.6%). Incorporation of protein N was 1.9 x that of carbohydrate and 3.2 x that of protein C, which indicates that the amino-N fraction of dietary protein was conserved (i.e. retained in tissues) relative to both carbohydrate and protein C. As much as 6% of absorbed protein C was excreted as dissolved organic matter, whereas only 1.7% was respired. These findings suggest that most dietary protein was completely broken down to satisfy the mussels' anabolic demand for amino N rather than catabolized for energy or retained as whole amino acids for anabolism (i.e. essential amino acids). Hence, the mussels appeared nutritionally limited by amino N rather than by energy or protein per se.
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Details
- Title
- Use of dual-labeled microcapsules to discern the physiological fates of assimilated carbohydrate, protein carbon, and protein nitrogen in suspension-feeding organisms
- Creators
- D A Kreeger - Drexel UniversityAJS HawkinsB L Bayne - Plymouth Marine Laboratory
- Publication Details
- Limnology and oceanography, v 41(2), pp 208-215
- Publisher
- AMER SOC LIMNOLOGY OCEANOGRAPH
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biodiversity, Earth, and Environmental Science (BEES)
- Web of Science ID
- WOS:A1996UK85000002
- Scopus ID
- 2-s2.0-0029783147
- Other Identifier
- 991019168727804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Limnology
- Oceanography