Journal article
Use of reverse-phase high-performance liquid chromatography in structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors
Analytical biochemistry, v 125(2), pp 395-405
1982
PMID: 7181098
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The neurophysins are a class of hypothalamo-neurohypophyseal proteins that function as carriers of the neuropeptide hormones oxytocin and vasopressin. Currently, we are using reverse-phase high-performance liquid chromatography for structural characterization of the neurophysins, their chemically modified derivatives, and biosynthetic precursors. A cyanopropylsilyl (Zorbax CN) matrix has been found to be efficient and convenient for separation of major tryptic peptides of performic acid, oxidized or reduced, and alkylated neurophysins. Using this peptide mapping system we have studied the site of modification of a photoaffinitylabeled derivative of bovine neurophysin II by separation and identification of covalently modified peptides. In addition, this system has been used for mapping subfemtomole amounts of radioactively labeled biosynthetic precursors of the neurophysins. This procedure has allowed identification of neurophysin sequences within both pre-pro-neurophysins produced by
in vitro translation and rat pro-neurophysins produced by
in vivo pulse labeling.
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Details
- Title
- Use of reverse-phase high-performance liquid chromatography in structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors
- Creators
- David M. Abercrombie - University of Maryland, College ParkChristopher J. Hough - National Institute of Diabetes and Digestive and Kidney DiseasesJonathan R. Seeman - National Institute of Diabetes and Digestive and Kidney DiseasesMichael J. Brownstein - National Institutes of HealthHarold Gainer - National Institute of Child Health and Human DevelopmentJames T. Russell - National Institute of Child Health and Human DevelopmentIrwin M. Chaiken - National Institute of Diabetes and Digestive and Kidney Diseases
- Publication Details
- Analytical biochemistry, v 125(2), pp 395-405
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Drexel University
- Web of Science ID
- WOS:A1982PN70900021
- Scopus ID
- 2-s2.0-0020372864
- Other Identifier
- 991019520529904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical