Journal article
Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120
Analytical and bioanalytical chemistry, v 396(3), pp 1143-1152
Feb 2010
PMID: 20016882
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We evaluated the potential of a quartz crystal microbalance with dissipation monitoring (QCM-D) to provide a sensitive, label-free method for detecting the conformational rearrangement of glycoprotein gp120 upon binding to different ligands. This glycoprotein is normally found on the envelope of the HIV-1 virus and is involved in viral entry into host cells. It was immobilized on the surface of the sensing element of the QCM-D and was exposed to individual solutions of several different small-molecule inhibitors as well as to a solution of soluble form of the host cell receptor to which gp120 binds. Instrument responses to ligand-triggered changes were in qualitative agreement with conformational changes suggested by other biophysical methods.
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Details
- Title
- Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120
- Creators
- Hyun-Su Lee - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USAMark Contarino - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15M Umashankara - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15Arne Schön - Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USAErnesto Freire - Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USAAmos B Smith - Department of Chemistry, University of Pennsylvania, 231 S. 34Irwin M Chaiken - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15Lynn S Penn - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA
- Publication Details
- Analytical and bioanalytical chemistry, v 396(3), pp 1143-1152
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Chemistry
- Web of Science ID
- WOS:000273845200020
- Scopus ID
- 2-s2.0-76749132713
- Other Identifier
- 991014877709404721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical