Journal article
Using siRNA to study microtubule-related proteins in cultured neurons
Methods in cell biology, v 131
2016
PMID: 26794513
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Testing functional hypotheses on the roles played by individual microtubule-related proteins in developing neurons benefits from having an effective means for depleting an individual protein from a population of cultured vertebrate neurons over an appropriate window. Small interfering RNA (siRNA) has the advantage of high transfection efficiency, but has certain limitations that require strategic experimental design. Here we describe the insights that we have gained over the years from using this approach.
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Details
- Title
- Using siRNA to study microtubule-related proteins in cultured neurons
- Creators
- Lanfranco Leo - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USAWenqian Yu - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USAPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USA
- Publication Details
- Methods in cell biology, v 131
- Publisher
- Elsevier; United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000437091900008
- Scopus ID
- 2-s2.0-84940689079
- Other Identifier
- 991014877857304721
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- Web of Science research areas
- Cell Biology