Journal article
Validation of a High-Performance Liquid Chromatography Separation of Weak Acids, Neutral Compounds, and Permanent Ions Using Sequential Elution with Tandem-Column Liquid Chromatography
Journal of separation science, v 48(3), e70124
Mar 2025
PMID: 40114433
Abstract
Sequential elution-liquid chromatography (SE-LC) employs conventional high-performance LC instrumentation to separate compounds by class and within each class using two or more sequential elution modes. The aim of this work was to validate a novel SE-LC method used to separate weak acids, permanently charged ions (anions), and neutral compounds by applying first a low pH isocratic elution to elute the weak acids, then an acetonitrile gradient to elute the neutral compounds, and lastly a sodium methanesulfonate gradient to elute the anionic compounds using a tandem-column arrangement comprised of a C18 column and a strong anion exchange column. The effects of flow rate, starting mobile phase organic content, column temperature, mobile phase composition, and gradient start time were investigated using a 2
fractional factorial design with center points for robustness. The method was demonstrated to be robust, specific, linear, accurate, and precise (repeatable) over the range of 6%-120% of the target analyte concentrations.
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Details
- Title
- Validation of a High-Performance Liquid Chromatography Separation of Weak Acids, Neutral Compounds, and Permanent Ions Using Sequential Elution with Tandem-Column Liquid Chromatography
- Creators
- Lauren Kline Lovejoy - Drexel UniversityJoe P Foley (Corresponding Author) - Drexel UniversityKaitlin M Grinias - GlaxoSmithKline (United States)
- Publication Details
- Journal of separation science, v 48(3), e70124
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- GSK RD
L.K.L. would like to thank GSK R&D, for the financial and material support received for this project. Also, L.K.L. would like to thank Kevin Owens for his feedback on the fractional factorial experimental design for the robustness study.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:001449451900001
- Scopus ID
- 2-s2.0-105001027303
- Other Identifier
- 991022041601304721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical