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Journal article
Varying Modulation of HIV-1 LTR Activity by BAF Complexes
Journal of molecular biology, v 411(3), pp 581-596
19 Aug 2011
PMID: 21699904
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The human immunodeficiency virus type 1 (HIV-1) long terminal repeat is present on both ends of the integrated viral genome and contains regulatory elements needed for transcriptional initiation and elongation. Post-integration, a highly ordered chromatin structure consisting of at least five nucleosomes, is found at the 5' long terminal repeat, the location and modification state of which control the state of active viral replication as well as silencing of the latent HIV-1 provirus. In this context, the chromatin remodeling field rapidly emerges as having a critical role in the control of viral gene expression. In the current study, we focused on unique Baf subunits that are common to the most highly recognized of chromatin remodeling proteins, the SWI/SNF (switching-defective-sucrose non-fermenting) complexes. We find that at least two Baf proteins, Baf53 and Baf170, are highly regulated in HIV-1-infected cells. Previously, studies have shown that the depletion of Baf53 in uninfected cells leads to the expansion of chromosomal territories and the decompaction of the chromatin. Baf53, in the presence of HIV-1 infection, co-elutes off of a chromatographic column as a different-sized complex when compared to uninfected cells and appears to be predominantly phosphorylated. The innate function of Baf53-containing complexes appears to be transcriptionally suppressive, in that knocking down Baf53 increases viral gene expression from cells both transiently and chronically infected with HIV-1. Additionally, cdk9/cyclin T in the presence of Tat is able to phosphorylate Baf53 in vitro, implying that this posttranslationally modified form relieves the suppressive effect and allows for viral transcription to proceed. (c) 2011 Elsevier Ltd. All rights reserved.
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Details
- Title
- Varying Modulation of HIV-1 LTR Activity by BAF Complexes
- Creators
- Rachel Van Duyne - Washington University Medical CenterIrene Guendel - George Mason UniversityAarthi Narayanan - George Mason UniversityEdward Gregg - George Mason UniversityNazly Shafagati - George Mason UniversityMudit Tyagi - George Mason UniversityRebecca Easley - George Mason UniversityZachary Klase - National Institute of Allergy and Infectious DiseasesSergei Nekhai - Howard UniversityKylene Kehn-Hall - George Mason UniversityFatah Kashanchi - George Mason University
- Publication Details
- Journal of molecular biology, v 411(3), pp 581-596
- Publisher
- Elsevier
- Number of pages
- 16
- Grant note
- AI078859; AI074410; AI043894 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R21AI074410 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000293938500007
- Scopus ID
- 2-s2.0-79960911664
- Other Identifier
- 991021902597904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology