Journal article
Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro
The American journal of pathology, v 145(5), pp 1023-1029
01 Nov 1994
PMID: 7526691
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The purpose of this study was to evaluate the vasoformative response of isolated vascular explants to a variety of growth factors that have been shown to stimulate angiogenesis. Rings of rat aorta were cultured in collagen gels under serum-free conditions in the presence or absence of vascular endothelial growth factor (VEGF), natural platelet-derived growth factor (PDGF), PDGF-AA, PDGF-BB, insulin-like growth factor-1 (IGF-1), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta 1 (TGF-beta 1), epidermal growth factor (EGF), interleukin-1 alpha (IL-1 alpha), or hepatocyte growth factor (HGF). The angiogenic response of the rat aorta was stimulated by VEGF, PDGF, PDGF-AA, PDGF-BB, and IGF-1. Maximum stimulatory effects were obtained with VEGF and PDGF-BB. By contrast, TGF-beta 1 and IL-1 alpha had inhibitory activity. No significant effects were observed with TGF-alpha, EGF, or HGF. The vascular outgrowth of VEGF-stimulated cultures was primarily composed of microvessels, whereas that of PDGF- and IGF-1-stimulated cultures contained an increased number of fibroblast-like cells. The inability of TGF-alpha, TGF-beta 1, IL-1 alpha, EGF, and HGF to stimulate rat aortic angiogenesis in serum-free culture suggests that either these factors require the mediatory activity of accessory cells that are not present in the rat aorta model or that blood vessels are heterogeneous in their capacity to respond to different angiogenic factors.
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Details
- Title
- Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro
- Creators
- R. F. Nicosia - Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129S. V. Nicosia - Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129M. Smith - Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129
- Publication Details
- The American journal of pathology, v 145(5), pp 1023-1029
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1994PQ08600006
- Scopus ID
- 2-s2.0-0028139239
- Other Identifier
- 991019184185004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Pathology