Journal article
Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons
Biofabrication, v 17(1)
18 Oct 2024
PMID: 39423834
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The gold standard to measure arterial health is vasodilation in response to nitric oxide (NO). Vasodilation is generally measured via pressure myography of arteries isolated from animal models. However, animal arteries can be difficult to obtain and may have limited relevance to human physiology. It is, therefore, critical to engineer human cell-based arterial models capable of contraction. Vascular smooth muscle cells (SMCs) must be circumferentially aligned around the vessel lumen to contract the vessel, which is challenging to achieve in a soft blood vessel model. In this study, we used gelatin microribbons to circumferentially align SMCs inside a hydrogel channel. To accomplish this, we created tunable gelatin microribbons of varying stiffnesses and thicknesses and assessed how SMCs aligned along them. We then wrapped soft, thick microribbons around a needle and encapsulated them in a gelatin methacryloyl hydrogel, forming a microribbon-lined channel. Finally, we seeded SMCs inside the channel and showed that they adhered best to fibronectin and circumferentially aligned in response to the microribbons. Together, these data show that tunable gelatin microribbons can be used to circumferentially align SMCs inside a channel. This technique can be used to create a human artery-on-a-chip to assess vasodilation via pressure myography, as well as to align other cell types for 3D in vitro models.
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Details
- Title
- Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons
- Creators
- Yusuf Mastoor - University of Maryland, College ParkMahsa Karimi - Drexel UniversityMichael Sun - University of Maryland, College ParkFereshteh Ahadi - Drexel UniversityPattie Mathieu - University of Maryland, College ParkMingyue Fan - Drexel UniversityLin Han - Drexel UniversityLi-Hsin Han - Drexel UniversityAlisa Morss-Clyne (Corresponding Author) - University of Maryland, College Park
- Publication Details
- Biofabrication, v 17(1)
- Publisher
- IOP Publishing Ltd; BRISTOL
- Number of pages
- 13
- Grant note
- National Institutes of Healthhttp://dx.doi.org/10.13039/100000002: R21EB028466 National Institutes of Health: CBET-1916997, CMMI-1751898 National Science Foundation
This work was supported by the National Institutes of Health R21EB028466 and the National Science Foundation CBET-1916997 and CMMI-1751898.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001346068000001
- Scopus ID
- 2-s2.0-85208204022
- Other Identifier
- 991021930864904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials