Journal article
Vascularized Brain Assembloids With Enhanced Cellular Complexity Provide Insights Into the Cellular Deficits of Tauopathy
Stem cells (Dayton, Ohio), v 42(2), pp 107-115
23 Nov 2023
PMID: 37995336
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Advanced technologies have enabled the engineering of self-organized 3-dimensional (3D) cellular structures from human induced pluripotent stem cells (hiPSCs), namely organoids, which recapitulate some key features of tissue development and functions of the human central nervous system (CNS). While hiPSC-derived 3D CNS organoids hold promise in providing a human-specific platform for studying CNS development and diseases, most of them do not incorporate the full range of implicated cell types, including vascular cell components and microglia, limiting their ability to accurately recreate the CNS environment and their utility in the study of certain aspects of the disease. Here we have developed a novel approach, called vascularized brain assembloids, for constructing hiPSC-derived 3D CNS structures with a higher level of cellular complexity. This is achieved by integrating forebrain organoids with common myeloid progenitors and phenotypically stabilized human umbilical vein endothelial cells (VeraVecs), which can be cultured and expanded in serum-free conditions. Compared with organoids, these assembloids exhibited enhanced neuroepithelial proliferation, advanced astrocytic maturation, and increased synapse numbers. Strikingly, the assembloids derived from hiPSCs harboring the tauP301S mutation exhibited increased levels of total tau and phosphorylated tau, along with a higher proportion of rod-like microglia-like cells and enhanced astrocytic activation, when compared to the assembloids derived from isogenic hiPSCs. Additionally, the tauP301S assembloids showed an altered profile of neuroinflammatory cytokines. This innovative assembloid technology serves as a compelling proof-of-concept model, opening new avenues for unraveling the intricate complexities of the human brain and accelerating progress in the development of effective treatments for neurological disorders.
Vascularized forebrain assembloids derived from the tauP301S mutant hiPSCs display elevated levels of total tau, phosphorylated tau, reactivated astrocytes, and distinctive pro-inflammatory cytokine profiles in contrast to their isogenic control counterparts. This innovative model holds significant promise for delving into the intricacies of the human brain and advancing our understanding of various neurological disorders.
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Details
- Title
- Vascularized Brain Assembloids With Enhanced Cellular Complexity Provide Insights Into the Cellular Deficits of Tauopathy
- Creators
- Xiaohuan Sun - Drexel UniversitySimeon Kofman - Drexel UniversityVictor C. Ogbolu - Drexel UniversityCeleste M. Karch - Washington University in St. LouisLarisa Ibric - Drexel UniversityLiang Qiang (Corresponding Author) - Drexel University
- Publication Details
- Stem cells (Dayton, Ohio), v 42(2), pp 107-115
- Publisher
- Oxford University Press
- Number of pages
- 9
- Grant note
- Angiocrine Inc. Lisa Dean Moseley Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; Pharmacology and Physiology; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001112289500001
- Scopus ID
- 2-s2.0-85184729503
- Other Identifier
- 991021811617404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Cell & Tissue Engineering
- Cell Biology
- Hematology
- Oncology