Journal article
Vertebrate Fidgetin Restrains Axonal Growth by Severing Labile Domains of Microtubules
Cell reports (Cambridge), v 12(11), pp 1723-1730
22 Sep 2015
PMID: 26344772
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Individual microtubules (MTs) in the axon consist of a stable domain that is highly acetylated and a labile domain that is not. Traditional MT-severing proteins preferentially cut the MT in the stable domain. In Drosophila, fidgetin behaves in this fashion, with targeted knockdown resulting in neurons with a higher fraction of acetylated (stable) MT mass in their axons. Conversely, in a fidgetin knockout mouse, the fraction of MT mass that is acetylated is lower than in the control animal. When fidgetin is depleted from cultured rodent neurons, there is a 62% increase in axonal MT mass, all of which is labile. Concomitantly, there are more minor processes and a longer axon. Together with experimental data showing that vertebrate fidgetin targets unacetylated tubulin, these results indicate that vertebrate fidgetin (unlike its fly ortholog) regulates neuronal development by tamping back the expansion of the labile domains of MTs.
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Details
- Title
- Vertebrate Fidgetin Restrains Axonal Growth by Severing Labile Domains of Microtubules
- Creators
- Lanfranco Leo - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USAWenqian Yu - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USAMitchell D'Rozario - Department of Biology, Drexel University, Philadelphia, PA 19104, USAEdward A Waddell - Department of Biology, Drexel University, Philadelphia, PA 19104, USADaniel R Marenda - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA; Department of Biology, Drexel University, Philadelphia, PA 19104, USAMichelle A Baird - National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FL 32310, USAMichael W Davidson - National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FL 32310, USABin Zhou - Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USABingro Wu - Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USALisa Baker - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USADavid J Sharp - Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USAPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA. Electronic address: pbaas@drexelmed.edu
- Publication Details
- Cell reports (Cambridge), v 12(11), pp 1723-1730
- Publisher
- Elsevier; United States
- Grant note
- R01 GM109909 / NIGMS NIH HHS R01 NS028785 / NINDS NIH HHS R01 NS28785 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; Neurobiology and Anatomy
- Web of Science ID
- WOS:000361617300002
- Scopus ID
- 2-s2.0-84942821536
- Other Identifier
- 991014878011904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology