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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics using Resolvable Poly(ethylene glycol) Mass Tags
Current protocols in chemical biology, Vol.5(4), pp.281-302
01 Dec 2013
PMCID: PMC3931299
PMID: 24391098
Abstract
O
-GlcNAc glycosylation is a dynamic protein posttranslational modification with roles in processes such as transcription, cell cycle regulation, and metabolism. Detailed mechanistic studies of
O
-GlcNAc have been hindered by a lack of methods for measuring
O
-GlcNAc stoichiometries and the interplay of glycosylation with other posttranslational modifications. We recently developed a method for labeling
O
-GlcNAc-modified proteins with resolvable poly(ethylene glycol) mass tags. This mass tagging approach enables the direct measurement of glycosylation stoichiometries and the visualization of distinct
O
-GlcNAc-modified subpopulations. Here, we describe protocols for labeling
O
-GlcNAc glycoproteins in cell lysates with mass tags.
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Details
- Title
- Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics using Resolvable Poly(ethylene glycol) Mass Tags
- Creators
- Peter M. Clark - Howard Hughes Medical InstituteJessica E. Rexach - Howard Hughes Medical InstituteLinda C. Hsieh-Wilson - Howard Hughes Medical Institute
- Publication Details
- Current protocols in chemical biology, Vol.5(4), pp.281-302
- Publisher
- Wiley
- Grant note
- || HHMI_ / Howard Hughes Medical Institute :
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University; School of Biomedical Engineering, Science, and Health Systems
- Identifiers
- 991019356498404721