O
-GlcNAc glycosylation is a dynamic protein posttranslational modification with roles in processes such as transcription, cell cycle regulation, and metabolism. Detailed mechanistic studies of
O
-GlcNAc have been hindered by a lack of methods for measuring
O
-GlcNAc stoichiometries and the interplay of glycosylation with other posttranslational modifications. We recently developed a method for labeling
O
-GlcNAc-modified proteins with resolvable poly(ethylene glycol) mass tags. This mass tagging approach enables the direct measurement of glycosylation stoichiometries and the visualization of distinct
O
-GlcNAc-modified subpopulations. Here, we describe protocols for labeling
O
-GlcNAc glycoproteins in cell lysates with mass tags.
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Details
Title
Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics using Resolvable Poly(ethylene glycol) Mass Tags
Creators
Peter M. Clark - Howard Hughes Medical Institute
Jessica E. Rexach - Howard Hughes Medical Institute
Linda C. Hsieh-Wilson - Howard Hughes Medical Institute
Publication Details
Current protocols in chemical biology, v 5(4), pp 281-302
Publisher
Wiley
Grant note
|| HHMI_ / Howard Hughes Medical Institute :
Resource Type
Journal article
Language
English
Academic Unit
School of Biomedical Engineering, Science, and Health Systems; Drexel University