Journal article
Visualizing Chromatin Modifications in Isolated Nuclei
Methods in molecular biology (Clifton, N.J.), v 2175, pp 23-31
2020
PMID: 32681481
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Modifications in chromatin structure are traditionally monitored by biochemical assays that provide average measurements of static events in a population of cells. Microscopy provides a method by which single cells or nuclei can be observed. Traditionally, microscopy has been used to image the nucleus by the application of immunostaining to chemically fixed samples or the use of exogenously expressed fluorescent proteins. This method represents an approach to observe changes in endogenous proteins relating to chromatin structure in real time. Here we describe a method for isolating transcriptionally and enzymatically active nuclei from live cells and visualizing events using fluorescently labeled antibodies. This method allows the observation of real time changes in chromatin architecture and can be used to observe the effects of drugs on nuclei while under microscopic observation.
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Details
- Title
- Visualizing Chromatin Modifications in Isolated Nuclei
- Creators
- Yuan Li - MSDZachary Klase - Drexel UniversityLuca Sardo - MSD
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 2175, pp 23-31
- Publisher
- Springer Nature
- Grant note
- DP2 DA044550 / NIDA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000681366300004
- Scopus ID
- 2-s2.0-85088496323
- Other Identifier
- 1071607634; 9781071607633; 991019167129304721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Cell Biology