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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Visualizing the secondary and tertiary architectural domains of lnc RNA RepA
Nature chemical biology, v 13(3)
01 Mar 2017
PMID: 28068310
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Long noncoding RNAs (lncRNAs) are important for gene expression, but little is known about their structures. RepA is a 1.6-kb mouse lncRNA comprising the same sequence as the 59 region of Xist, including A and F repeats. It has been proposed to facilitate the initiation and spread of X-chromosome inactivation, although its exact role is poorly understood. To gain insight into the molecular mechanism of RepA and Xist, we determined a complete phylogenetically validated secondary-structural map of RepA through SHAPE and DMS chemical probing of a homogeneously folded RNA in vitro. We combined UV-cross-linking experiments with RNA modeling methods to produce a three-dimensional model of RepA functional domains demonstrating that tertiary architecture exists within lncRNA molecules and occurs within specific functional modules. This work provides a foundation for understanding of the evolution and functional properties of RepA and Xist and offers a framework for exploring architectural features of other lncRNAs.
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Details
- Title
- Visualizing the secondary and tertiary architectural domains of lnc RNA RepA
- Creators
- Fei Liu - Yale UniversitySrinivas Somarowthu - Yale UniversityAnna Marie Pyle - Yale University
- Publication Details
- Nature chemical biology, v 13(3)
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- R01GM050313 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01GM50313 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000394431500010
- Scopus ID
- 2-s2.0-85008618454
- Other Identifier
- 991019167323904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology