Journal article
Water sorption isotherms and enthalpies of water sorption by lysozyme using the quartz crystal microbalance/heat conduction calorimeter
Biochimica et biophysica acta, v 1594(1), pp 150-159
31 Jan 2002
PMID: 11825617
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The water sorption isotherm and the water vapor activity dependence of the enthalpy of water sorption Delta(sorp)Hdegrees of lysozyme have been measured at 25 degrees C. A thin film of lysozyme of mass 250 microg was exposed to H2O/N2 mixtures in a quartz crystal microbalance/heat conduction calorimeter (QCM/HCC). The QCM/HCC is a new gravimetric/calorimetric method that measures simultaneously and with high precision the mass change and the corresponding heat flow in a thin film exposed to a gas. Delta(sorp)Hdegrees for lysozyme agrees with previous determinations, although hysteresis effects are evident in the data. No van't Hoff analysis is necessary because sorption enthalpies are measured calorimetrically. The water vapor activity dependence of Delta(sorp)Hdegrees agrees with that measured previously by Bone. As the water content of the lysozyme film drops below 10 mass%, Delta(sorp)Hdegrees becomes more exothermic, indicating that water is being bound to the charged or highly polar groups of the solvent-accessible surface of lysozyme. The dynamics of water uptake and release from lysozyme thin films are much slower than in polymer films of comparable thickness. Because the QCM/HCC operates with sub-milligram samples, any protein is now amenable to study by this technique.
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Details
- Title
- Water sorption isotherms and enthalpies of water sorption by lysozyme using the quartz crystal microbalance/heat conduction calorimeter
- Creators
- Allan L Smith - Drexel UniversityHamid M Shirazi - Drexel UniversityS Rose Mulligan - Drexel UniversityMichelle J Dolinski - Physics
- Publication Details
- Biochimica et biophysica acta, v 1594(1), pp 150-159
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000173826100015
- Scopus ID
- 2-s2.0-0037203805
- Other Identifier
- 991019186653704721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics