Journal article
Whole-body fluorescent imaging of GFP-expressing tumors
Shēngwù huàxué yŭ shēngwù wùlĭ jìnzhán, Vol.29(6), pp.942-945
01 Dec 2002
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human lung cancer cells (ASTC-a-1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. The flow cytometry was used to determine the GFP expression stability, the results showed that there was significant difference between 2 # (3 #) and 4 # (5 #) (P<0.01). The nude mouse, 6 weeks of age, was injected s. c. with a single dose of 3x10(6) infected tumor cells. The GFP fluorescence was directly exited by 488 nm argon ion laser and recorded by digital camera with 530 nm long pass filter.
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Details
- Title
- Whole-body fluorescent imaging of GFP-expressing tumors
- Creators
- Y JinD Xing
- Publication Details
- Shēngwù huàxué yŭ shēngwù wùlĭ jìnzhán, Vol.29(6), pp.942-945
- Publisher
- SCIENCE CHINA PRESS
- Number of pages
- 4
- Resource Type
- Journal article
- Language
- Chinese
- Academic Unit
- Neurobiology and Anatomy
- Identifiers
- 991020545119704721
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- Biochemistry & Molecular Biology
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