Logo image
Back
Whole genome sequencing of Streptomyces actuosus ISP-5337, Streptomyces sioyaensis B-5408, and Actinospica acidiphila B-2296 reveals secondary metabolomes with antibiotic potential
Journal article   Open access   Peer reviewed

Whole genome sequencing of Streptomyces actuosus ISP-5337, Streptomyces sioyaensis B-5408, and Actinospica acidiphila B-2296 reveals secondary metabolomes with antibiotic potential

Haley M Majer, Rachel L Ehrlich, Azad Ahmed, Joshua P Earl, Garth D Ehrlich and Joris Beld
Biotechnology reports (Amsterdam, Netherlands), v 29, pp e00596-e00596
Mar 2021
DOI: https://doi.org/10.1016/j.btre.2021.e00596
PMID: 33643857
url
https://doi.org/10.1016/j.btre.2021.e00596View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

AntiSMASH Whole genome sequencing Mass spectrometry Thiopeptides
are bacteria of biotechnological importance since they are avid producers of secondary metabolites, including antibiotics. Progress in genome mining has recently shown that species encode for many biosynthetic gene clusters which are mostly unexplored. Here, we selected three Actinomycetes species for whole genome sequencing that are known to produce potent thiopeptide antibiotics. biosynthesizes nosiheptide, produces siomycin, and is a member of the subfamily. Bioinformatic analyses demonstrated diverse secondary metabolomes with multiple antibiotic-encoding gene clusters. Detailed mass spectrometry analysis of metabolite extracts verified the active expression of nosiheptide and siomycin from and while fractionation of the bacterial extracts and subsequent challenge against demonstrated potent antibiotic activity of fractions containing these compounds. Whole genome sequencing of these species facilitates future bioengineering efforts for thiopeptides and characterization of relevant secondary metabolites.

Metrics

18 Record Views
16 citations in Scopus

Details

Logo image