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Journal article
Winner of the Young Investigator Award of the Society for Biomaterials (USA) for 2016, 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Aligned microribbon-like hydrogels for guiding three-dimensional smooth muscle tissue regeneration
Journal of biomedical materials research. Part A, v 104(5), pp 1064-1071
01 May 2016
PMID: 26799256
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Smooth muscle tissue is characterized by aligned structures, which is critical for its contractile functions. Smooth muscle injury is common and can be caused by various diseases and degenerative processes, and there remains a strong need to develop effective therapies for smooth muscle tissue regeneration with restored structures. To guide cell alignment, previously cells were cultured on 2D nano/microgrooved substrates, but such method is limited to fabricating 2D aligned cell sheets only. Alternatively, aligned electrospun nanofiber has been employed as 3D scaffold for cell alignment, but cells can only be seeded post fabrication, and nanoporosity of electrospun fiber meshes often leads to poor cell distribution. To overcome these limitations, we report aligned gelatin-based microribbons (mu RBs) as macroporous hydrogels for guiding smooth muscle alignment in 3D. We developed aligned mu RB-like hydrogels using wet spinning, which allows easy fabrication of tissue-scale (cm) macroporous matrices with alignment cues and supports direct cell encapsulation. The macroporosity within mu RB-based hydrogels facilitated cell proliferation, new matrix deposition, and nutrient diffusion. In aligned mu RB scaffold, smooth muscle cells showed high viability, rapid adhesion, and alignment following mu RB direction. Aligned mu RB scaffolds supported retention of smooth muscle contractile phenotype, and accelerated uniaxial deposition of new matrix (collagen I/IV) along the mu RB. In contrast, cells encapsulated in conventional gelatin hydrogels remained round with matrix deposition limited to pericellular regions only. We envision such aligned mu RB scaffold can be broadly applicable in growing other anisotropic tissues including tendon, nerves and blood vessel. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1064-1071, 2016.
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Details
- Title
- Winner of the Young Investigator Award of the Society for Biomaterials (USA) for 2016, 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Aligned microribbon-like hydrogels for guiding three-dimensional smooth muscle tissue regeneration
- Creators
- Soah Lee - Stanford UniversityXinming Tong - Stanford UniversityLi-Hsin Han - Stanford UniversityAnthony Behn - Stanford UniversityFan Yang - Stanford University
- Publication Details
- Journal of biomedical materials research. Part A, v 104(5), pp 1064-1071
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- Stanford Chem-H Institute R01DE0,2,4772 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA TR3-05,569 / California Institute for Regenerative Medicine Early Translational III Grant R01DE024772 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) RT3-07,804 / California Institute for Regenerative Medicine Tools and Technologies Development Grant CBET-13,51 289 / Bio-X Interdisciplinary Initiatives Program Grant, National Science Foundation CAREER award program
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Drexel University
- Web of Science ID
- WOS:000373718300002
- Scopus ID
- 2-s2.0-84975784267
- Other Identifier
- 991020100050604721
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- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials