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Journal article
Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Microribbon-based hydrogels accelerate stem cell-based bone regeneration in a mouse critical-size cranial defect model
Journal of biomedical materials research. Part A, v 104(6), pp 1321-1331
01 Jun 2016
PMID: 26991141
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Stem cell-based therapies hold great promise for enhancing tissue regeneration. However, the majority of cells die shortly after transplantation, which greatly diminishes the efficacy of stem cell-based therapies. Poor cell engraftment and survival remain a major bottleneck to fully exploiting the power of stem cells for regenerative medicine. Biomaterials such as hydrogels can serve as artificial matrices to protect cells during delivery and guide desirable cell fates. However, conventional hydrogels often lack macroporosity, which restricts cell proliferation and delays matrix deposition. Here we report the use of injectable, macroporous microribbon (RB) hydrogels as stem cell carriers for bone repair, which supports direct cell encapsulation into a macroporous scaffold with rapid spreading. When transplanted in a critical-sized, mouse cranial defect model, RB-based hydrogels significantly enhanced the survival of transplanted adipose-derived stromal cells (ADSCs) (81%) and enabled up to three-fold cell proliferation after 7 days. In contrast, conventional hydrogels only led to 27% cell survival, which continued to decrease over time. MicroCT imaging showed RBs enhanced and accelerated mineralized bone repair compared to hydrogels (61% vs. 34% by week 6), and stem cells were required for bone repair to occur. These results suggest that paracrine signaling of transplanted stem cells are responsible for the observed bone repair, and enhancing cell survival and proliferation using RBs further promoted the paracrine-signaling effects of ADSCs for stimulating endogenous bone repair. We envision RB-based scaffolds can be broadly useful as a novel scaffold for enhancing stem cell survival and regeneration of other tissue types. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1321-1331, 2016.
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Details
- Title
- Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Microribbon-based hydrogels accelerate stem cell-based bone regeneration in a mouse critical-size cranial defect model
- Creators
- Li-Hsin Han - Stanford UniversityBogdan Conrad - Stanford UniversityMichael T. Chung - Stanford UniversityLorenzo Deveza - Stanford UniversityXinyi Jiang - Stanford UniversityAndrew Wang - Stanford UniversityManish J. Butte - Stanford UniversityMichael T. Longaker - Stanford UniversityDerrick Wan - Stanford UniversityFan Yang - Stanford University
- Publication Details
- Journal of biomedical materials research. Part A, v 104(6), pp 1321-1331
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- 1264833 / Directorate For Engineering; National Science Foundation (NSF); NSF - Directorate for Engineering (ENG) R01DE024772 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01 DE024772 / NIDCR NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01GM110482 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01 GM110482 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; Drexel University
- Web of Science ID
- WOS:000375117200001
- Scopus ID
- 2-s2.0-84964328556
- Other Identifier
- 991020100183004721
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- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials