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Journal article
XBP-1, a Novel Human T-Lymphotropic Virus Type 1 (HTLV-1) Tax Binding Protein, Activates HTLV-1 Basal and Tax-Activated Transcription
Journal of virology, v 82(9), pp 4343-4353
01 May 2008
PMID: 18287238
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
X-box binding protein 1 (XBP-1), a basic leucine zipper transcription factor, plays a key role in the cellular unfolded protein response (UPR). There are two XBP-1 isoforms in cells, spliced XBP-1S and unspliced XBP-1U. XBP-1U has been shown to bind to the 21-bp Tax-responsive element of the human T-lymphotropic virus type 1 (HTLV-1) long terminal repeat (LTR) in vitro and transactivate HTLV-1 transcription. Here we identify XBP-1S as a transcription activator of HTLV-1. Compared to XBP-1U, XBP-1S demonstrates stronger activating effects on both basal and Tax-activated HTLV-1 transcription in cells. Our results show that both XBP-1S and XBP-1U interact with Tax and bind to the HTLV-1 LTR in vivo. In addition, elevated mRNA levels of the gene for XBP-1 and several UPR genes were detected in the HTLV-1-infected C10/MJ and MT2 T-cell lines, suggesting that HTLV-1 infection may trigger the UPR in host cells. We also identify Tax as a positive regulator of the expression of the gene for XBP-1. Activation of the UPR by tunicamycin showed no effect on the HTLV-1 LTR, suggesting that HTLV-1 transcription is specifically regulated by XBP-1. Collectively, our study demonstrates a novel host-virus interaction between a cellular factor XBP-1 and transcriptional regulation of HTLV-1.
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Details
- Title
- XBP-1, a Novel Human T-Lymphotropic Virus Type 1 (HTLV-1) Tax Binding Protein, Activates HTLV-1 Basal and Tax-Activated Transcription
- Creators
- Sebastian C. Y. Ku - Bioprocessing Technology InstituteJialing Lee - Bioprocessing Technology InstituteJoanne Lau - Bioprocessing Technology InstituteMeera Gurumurthy - Bioprocessing Technology InstituteRaymond Ng - George Washington UniversitySiew Hui Lwa - Bioprocessing Technology InstituteJoseph Lee - Bioprocessing Technology InstituteZachary Klase - George Washington UniversityFatah Kashanchi - George Washington UniversitySheng-Hao Chao - George Washington University
- Publication Details
- Journal of virology, v 82(9), pp 4343-4353
- Publisher
- American Society for Microbiology (ASM)
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000255084600015
- Scopus ID
- 2-s2.0-42449154243
- Other Identifier
- 991021902505104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology