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Published, Version of Record (VoR)CC BY-NC-SA V4.0, Open
Journal article
YAP and TAZ regulate cell volume
The Journal of cell biology, v 218(10), pp 3472-3488
07 Oct 2019
PMID: 31481532
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty in accurately quantifying cell volume in a high-throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are regulators of single-cell volume. The role of YAP/TAZ in volume regulation must go beyond its influence on total cell cycle duration or cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find that YAP/TAZ directly impacts the cell division volume, and YAP is involved in regulating intracellular cytoplasmic pressure. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibiting myosin assembly and cell tension slows cell cycle progression from G1 to S. These results suggest that YAP/TAZ may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.
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Details
- Title
- YAP and TAZ regulate cell volume
- Creators
- Nicolas A Perez-Gonzalez - Johns Hopkins UniversityNash D Rochman - Johns Hopkins UniversityKai Yao - Department of Mechanical Engineering Johns Hopkins University Baltimore MDJiaxiang Tao - Department of Mechanical Engineering Johns Hopkins University Baltimore MDMinh-Tam Tran Le - Johns Hopkins UniversityShannon Flanary - Johns Hopkins UniversityLucia Sablich - Johns Hopkins UniversityBen Toler - Johns Hopkins UniversityEliana Crentsil - Johns Hopkins UniversityFelipe Takaesu - Johns Hopkins UniversityBram Lambrus - Johns Hopkins MedicineJessie Huang - Department of Environmental Health and Engineering Johns Hopkins Bloomberg School of Public Health Baltimore MD.Vivian Fu - University of California, San DiegoPragati Chengappa - Department of Biology Drexel University Philadelphia PATia M Jones - Department of Biology Drexel University Philadelphia PAAndrew J Holland - Johns Hopkins MedicineSteven An - Department of Environmental Health and Engineering Johns Hopkins Bloomberg School of Public Health Baltimore MD.Denis Wirtz - Johns Hopkins UniversityRyan J Petrie - Drexel UniversityKun-Liang Guan - University of California, San DiegoSean X Sun - Johns Hopkins University
- Publication Details
- The Journal of cell biology, v 218(10), pp 3472-3488
- Grant note
- U54 CA210173 / NCI NIH HHS T32 CA130840 / NCI NIH HHS R01 GM126054 / NIGMS NIH HHS T32 CA153952 / NCI NIH HHS T34 GM008663 / NIGMS NIH HHS R01 GM101464 / NIGMS NIH HHS R01 GM114119 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000489119800023
- Scopus ID
- 2-s2.0-85072993582
- Other Identifier
- 991019167766804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology