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Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses
Journal article   Open access   Peer reviewed

Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses

Denis Gaucher, Rene Therrien, Nadia Kettaf, Bastian R. Angermann, Genevieve Boucher, Abdelali Filali-Mouhim, Janice M. Moser, Riyaz S. Mehta, Donald R. Drake, Erika Castro, …
The Journal of experimental medicine, v 205(13), pp 3119-3131
22 Dec 2008
PMID: 19047440
url
https://rupress.org/jem/article-pdf/205/13/3119/1197243/jem_20082292.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1084/jem.20082292View
Published, Version of Record (VoR) Open

Abstract

Immunology Life Sciences & Biomedicine Medicine, Research & Experimental Research & Experimental Medicine Science & Technology
Correlates of immune-mediated protection to most viral and cancer vaccines are still unknown. This impedes the development of novel vaccines to incurable diseases such as HIV and cancer. In this study, we have used functional genomics and polychromatic flow cytometry to define the signature of the immune response to the yellow fever (YF) vaccine 17D (YF17D) in a cohort of 40 volunteers followed for up to 1 yr after vaccination. We show that immunization with YF17D leads to an integrated immune response that includes several effector arms of innate immunity, including complement, the inflammasome, and interferons, as well as adaptive immunity as shown by an early T cell response followed by a brisk and variable B cell response. Development of these responses is preceded, as demonstrated in three independent vaccination trials and in a novel in vitro system of primary immune responses ( modular immune in vitro construct [ MIMIC] system), by the coordinated up-regulation of transcripts for specific transcription factors, including STAT1, IRF7, and ETS2, which are upstream of the different effector arms of the immune response. These results clearly show that the immune response to a strong vaccine is preceded by coordinated induction of master transcription factors that lead to the development of a broad, polyfunctional, and persistent immune response that integrates all effector cells of the immune system.

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Industry collaboration
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Web of Science research areas
Immunology
Medicine, Research & Experimental
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