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Zinc ions and alkaline PH alter the phosphorylated state of proteins 3 and 4.2 in human erythrocyte membranes
Journal article   Peer reviewed

Zinc ions and alkaline PH alter the phosphorylated state of proteins 3 and 4.2 in human erythrocyte membranes

Richard L. Fennell and Gerald Soslau
Thrombosis research, v 66(6), pp 637-647
1992
PMID: 1519224

Abstract

phosphoprotein proteins 3 and 4.2 Red blood cell
The phosphorylation patterns of isolated red blood cell (RBC) membranes labeled with [ γ- 32p]ATP are altered by Zn ++ ions. Zn ++ ions caused an increased phosphate incorporation into a 72 KDa protein and several proteins in the 40–60 KDa region and a decrease in the labeling of a 53 KDa protein. The 72 KDa and 53 KDa proteins have been identified as protein 4.2 and a protease-cleaved fragment of protein 3, respectively. Evidence suggests that the changes in phosphorylation pattern may be due to the stimulation of endogenous membrane alkaline phosphatase(s). Our results suggest that Zn ++, at physiological concentrations in the intact erythrocyte, could modulate the phosphorylation of selected proteins which may regulate their association in the cytoskeletal network.

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Web of Science research areas
Hematology
Peripheral Vascular Disease
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