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cAMP promotes gap junctional coupling in T84 cells
Journal article   Peer reviewed

cAMP promotes gap junctional coupling in T84 cells

M Chanson, M M White and S S Garber
The American journal of physiology, v 271(2 Pt 1), pp C533-539
Aug 1996
DOI: https://doi.org/10.1152/ajpcell.1996.271.2.c533
PMID: 8769992
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Cell Line Colon - cytology Colon - physiology Connexins - metabolism Cyclic AMP - physiology Cyclic AMP-Dependent Protein Kinases - agonists Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors Electric Conductivity Electrophysiology Fluorescent Dyes Gap Junctions - physiology Humans Ion Channels - physiology Isoquinolines Osmolar Concentration
The effect of intracellular adenosine 3',5'-cyclic monophosphate (cAMP) on dye and electrical coupling was studied in T84 cells, a cell line often used as a model for epithelial cell fluid secretion. Injections of lucifer yellow (LY) into single cells within a cluster of control cells resulted in LY localization to 1.3 +/- 0.2 (mean +/- SE) cells within a cluster. Twenty-six percent of control T84 cell pairs were electrically coupled as assayed by the dual patch-clamp technique. Treatment of cells with agents that either increase intracellular cAMP and/or activate protein kinase A (PKA) increased dye localization to 3.8 +/- 0.6 cells and the proportion of electrically coupled cell pairs to 65%. No electrical coupling was observed in the presence of the Rp diastereomer of adenosine-3',5'-cyclic monophosphothioate (Rp-cAMPS), a PKA antagonist. Excess of Rp-cAMPS prevented cell coupling elicited by 20 microM of the Sp diastereomer of adenosine-3',5'-cyclic monophosphothioate. Expression of connexin 32 mRNA, but not of connexins 26, 43, or 45, was detected by reverse transcription-polymerase chain reaction. These results suggest that communication between T84 cells is modulated by PKA, providing a mechanism for regulating multicellular activity, such as fluid secretion.

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Web of Science research areas
Cell Biology
Physiology
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