Journal article
cDNA sequence and differential mRNA regulation of two forms of glial cell line-derived neurotrophic factor in Schwann cells and rat skeletal muscle
Experimental neurology, v 131(1), pp 47-52
1995
PMID: 7895811
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Total RNA from rat Schwann cells grown in culture and adult rat skeletal muscle was reverse transcribed, amplified for glial cell line-derived neurotrophic factor (GDNF) messenger RNA (mRNA) using the polymerase chain reaction (PCR), and the PCR products sequenced. Two forms of GDNF were detected in the PCR step, one of a predicted size (GDNF
633) and a second smaller form missing a 78-base pair sequence (GDNF
555). Sequence analysis demonstrated that GDNF
633 is similar to the published sequence of GDNF differing only at three nucleotides. Southern and Northern blot analyses reveal that the two forms are probably derived from a single RNA species that is alternatively spliced. Interestingly, (GDNF
633) mRNA was found to be selectively upregulated in denervated rat skeletal muscle at 1–2 weeks following axotomy, providing evidence that the innervation status of the muscle may determine the expression profile of the two alternately spliced forms. Given these findings, we suggest that GDNF may function as a target-derived trophic factor for neuronal populations innervating skeletal muscle, including sensory neurons and spinal cord motoneurons.
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Details
- Title
- cDNA sequence and differential mRNA regulation of two forms of glial cell line-derived neurotrophic factor in Schwann cells and rat skeletal muscle
- Creators
- Joe E. Springer - Drexel UniversityJeffrey L. Seeburger - Drexel UniversityH.E. Jin - Department of Anatomy and Neurobiology, Medical College of Pennsylvania and Hahnemann University, Philadelphia, Pennsylvania 19102, USAAna Gabrea - Drexel UniversityElizabeth P. Blankenhorn - Drexel UniversityLawrence W. Bergman - Drexel UniversityJialuo He - Civil, Architectural, and Environmental Engineering
- Publication Details
- Experimental neurology, v 131(1), pp 47-52
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:A1995QN05600005
- Scopus ID
- 2-s2.0-0028901367
- Other Identifier
- 991019184031704721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences