Files and links (1)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
gamma delta T Cells but Not NK Cells Are Essential for Cell-Mediated Immunity against Plasmodium chabaudi Malaria
Infection and immunity, v 78(10), pp 4331-4340
01 Oct 2010
PMID: 20660608
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Blood-stage Plasmodium chabaudi infections are suppressed by antibody-mediated immunity and/or cell-mediated immunity (CMI). To determine the contributions of NK cells and gamma delta T cells to protective immunity, C57BL/6 (wild-type [WT]) mice and B-cell-deficient (J(H-/-)) mice were infected with P. chabaudi and depleted of NK cells or gamma delta T cells with monoclonal antibody. The time courses of parasitemia in NK-cell-depleted WT mice and J(H-/-) mice were similar to those of control mice, indicating that deficiencies in NK cells, NKT cells, or CD8(+) T cells had little effect on parasitemia. In contrast, high levels of noncuring parasitemia occurred in J(H-/-) mice depleted of gamma delta T cells. Depletion of gamma delta T cells during chronic parasitemia in B-cell-deficient J(H-/-) mice resulted in an immediate and marked exacerbation of parasitemia, suggesting that gamma delta T cells have a direct killing effect in vivo on blood-stage parasites. Cytokine analyses revealed that levels of interleukin-10, gamma interferon (IFN-gamma), and macrophage chemoattractant protein 1 (MCP-1) in the sera of gamma delta T-cell-depleted mice were significantly (P < 0.05) decreased compared to hamster immunoglobulin-injected controls, but these cytokine levels were similar in NK-cell-depleted mice and their controls. The time courses of parasitemia in CCR2(-/-) and J(H-/-) x CCR2(-/-) mice and in their controls were nearly identical, indicating that MCP-1 is not required for the control of parasitemia. Collectively, these data indicate that the suppression of acute P. chabaudi infection by CMI is gamma delta T cell dependent, is independent of NK cells, and may be attributed to the deficient IFN-gamma response seen early in gamma delta T-cell-depleted mice.
Metrics
Details
- Title
- gamma delta T Cells but Not NK Cells Are Essential for Cell-Mediated Immunity against Plasmodium chabaudi Malaria
- Creators
- William P. Weidanz - University of Wisconsin–MadisonGayeLyn LaFleur - University of Wisconsin–MadisonAndrew Brown - University of Wisconsin–MadisonJames M. Burns - Drexel UniversityIrene Gramaglia - La Jolla Bioengineering InstituteHenri C. van der Heyde - La Jolla Bioengineering Institute
- Publication Details
- Infection and immunity, v 78(10), pp 4331-4340
- Publisher
- Amer Soc Microbiology
- Number of pages
- 10
- Grant note
- AI12710; AI49585; AI40667 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AI040667 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000282004200026
- Scopus ID
- 2-s2.0-77957728515
- Other Identifier
- 991019169037504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases