Journal article
is a subset of -Opioid Receptor Redistribution in the Locus Coeruleus Upon Precipitation of Withdrawal in Opiate-Dependent Rats
Anatomical record (Hoboken, N.J. : 2007), v 292(3), pp 401-411
01 Mar 2009
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Abstract
Administration of -opioid receptor (MOR) agonists is known to produce adaptive changes within noradrenergic neurons of the rat locus coeruleus (LC). Alterations in the subcellular distribution of MOR have been shown to occur in the LC in response to full agonists and endogenous peptides; however, there is considerable debate in the literature whether trafficking of MOR occurs after chronic exposure to the partial-agonist morphine. In the present study, we examined adaptations in MOR after chronic opioid exposure using immunofluorescence and electron microscopy (EM), using receptor internalization as a functional endpoint. MOR trafficking in LC neurons was characterized in morphine-dependent rats that were given naltrexone at a dose known to precipitate withdrawal. After chronic morphine exposure, a subtle redistribution of MOR immunoreactivity from the membrane to the cytosol was detected within dendrites of LC neurons. Interestingly, an acute injection of naltrexone in rats exposed to chronic morphine produced a robust internalization of MOR, whereas administration of naltrexone failed to do so in naive animals. These findings provide anatomical evidence for modified regulation of MOR trafficking after chronic morphine treatment in brain noradrenergic neurons. Adaptations in the MOR signaling pathways that regulate internalization may occur as a consequence of chronic treatment and precipitation of withdrawal. Mechanisms underlying this effect might include differential MOR regulation in the LC, or downstream effects of withdrawal-induced enkephalin (ENK) release from afferents to the LC. Anat Rec, 292:401-411, 2009.
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Details
- Title
- is a subset of -Opioid Receptor Redistribution in the Locus Coeruleus Upon Precipitation of Withdrawal in Opiate-Dependent Rats
- Creators
- Jillian Scavone - Thomas Jefferson UniversityElisabeth Van Bockstaele - Thomas Jefferson University
- Publication Details
- Anatomical record (Hoboken, N.J. : 2007), v 292(3), pp 401-411
- Publisher
- WILEY; HOBOKEN
- Number of pages
- 11
- Grant note
- NIH: DA 09082, DA 15123, DA 15395, DA 023755, EY 014798
Grant sponsor: NIH grants; Grant numbers: DA 09082, 15123, 15395, 023755 and EY 014798.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000263983400013
- Scopus ID
- 2-s2.0-65549119356
- Other Identifier
- 991021903291304721
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- Web of Science research areas
- Anatomy & Morphology