Journal article
mRNAs and Protein Synthetic Machinery Localize into Regenerating Spinal Cord Axons When They Are Provided a Substrate That Supports Growth
The Journal of neuroscience, v 35(28), pp 10357-10370
15 Jul 2015
PMID: 26180210
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although intra-axonal protein synthesis is well recognized in cultured neurons and during development in vivo, there have been few reports of mRNA localization and/or intra-axonal translation in mature CNS axons. Indeed, previous work indicated that mature CNS axons contain much lower quantities of translational machinery than PNS axons, leading to the conclusion that the capacity for intra-axonal protein synthesis is linked to the intrinsic capacity of a neuron for regeneration, with mature CNS neurons showing much less growth after injury than PNS neurons. However, when regeneration by CNS axons is facilitated, it is not known whether the intra-axonal content of translational machinery changes or whether mRNAs localize into these axons. Here, we have used a peripheral nerve segment grafted into the transected spinal cord of adult rats as a supportive environment for regeneration by ascending spinal axons. By quantitative fluorescent in situ hybridization combined with immunofluorescence to unambiguously distinguish intra-axonalmRNAs, we show that regenerating spinal cord axons contain beta-actin, GAP-43, Neuritin, Reg3a, Hamp, and Importin beta 1 mRNAs. These axons also contain 5S rRNA, phosphorylated S6 ribosomal protein, eIF2 alpha translation factor, and 4EBP1 translation factor inhibitory protein. Different levels of these mRNAs in CNS axons from regenerating PNS axons may relate to differences in the growth capacity of these neurons, although the presence of mRNA transport and likely local translation in both CNS and PNS neurons suggests an active role in the regenerative process.
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Details
- Title
- mRNAs and Protein Synthetic Machinery Localize into Regenerating Spinal Cord Axons When They Are Provided a Substrate That Supports Growth
- Creators
- Ashley L. Kalinski - Drexel UniversityRahul Sachdeva - Drexel UniversityCynthia Gomes - University of South CarolinaSeung Joon Lee - University of South CarolinaZalak Shah - University of South CarolinaJohn D. Houle - Drexel UniversityJeffery L. Twiss - Drexel University
- Publication Details
- The Journal of neuroscience, v 35(28), pp 10357-10370
- Publisher
- Soc Neuroscience
- Number of pages
- 14
- Grant note
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation R01NS041596 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) South Carolina SmartState Endowment Program (Center for Childhood Neurotherapeutics) through the University of South Carolina 224125 / Craig H. Nielsen Foundation P01-NS055976 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000358298500024
- Scopus ID
- 2-s2.0-84937597364
- Other Identifier
- 991019168890604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences