Journal article
microRNA-200b and microRNA-200c promote colorectal cancer cell proliferation via targeting the reversion-inducing cysteine-rich protein with Kazal motifs
RNA biology, v 12(3), pp 276-289
01 Mar 2015
PMID: 25826661
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
MicroRNA-200b and microRNA-200c (miR-200b/c) are 2 of the most frequently upregulated oncomiRs in colorectal cancer cells. The role of miR-200b/c during colorectal tumorigenesis, however, remains unclear. In the present study, we report that miR-200b/c can promote colorectal cancer cell proliferation via targeting the reversion-inducing cysteine-rich protein with Kazal motifs (RECK). Firstly, bioinformatics analysis predicted RECK as a conserved target of miR-200b/c. By overexpressing or knocking down miR-200b/c in colorectal cancer cells, we experimentally validated that miR200b/c are direct regulators of RECK. Secondly, an inverse correlation between the levels of miR-200b/c and RECK protein was found in human colorectal cancer tissues and cell lines. Thirdly, we demonstrated that repression of RECK by miR-200b/c consequently triggered SKP2 (S-phase kinase-associated protein 2) elevation and p27(Kip1) (also known as cyclin-dependent kinase inhibitor 1B) degradation in colorectal cancer cells, which eventually promotes cancer cell proliferation. Finally, promoting tumor cell growth by miR-200b/c-targeting RECK was also observed in the xenograft mouse model. Taken together, our results demonstrate that miR-200b/c play a critical role in promoting colorectal tumorigenesis through inhibiting RECK expression and subsequently triggering SKP2 elevation and p27(Kip1) degradation.
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Details
- Title
- microRNA-200b and microRNA-200c promote colorectal cancer cell proliferation via targeting the reversion-inducing cysteine-rich protein with Kazal motifs
- Creators
- Yi Pan - Jiangsu UniversityHongwei Liang - Pharmaceutical Biotechnology (Czechia)Weixu Chen - College Station Medical CenterHongjie Zhang - Nanjing UniversityNan Wang - Nanjing UniversityFeng Wang - Nanjing Drum Tower HospitalSuyang Zhang - Nanjing UniversityYanqing Liu - Nanjing UniversityChihao Zhao - Nanjing UniversityXin Yan - Nanjing UniversityJunfeng Zhang - Nanjing UniversityChen-Yu Zhang - Nanjing UniversityHongwei Gu - Nanjing UniversityKe Zen - Nanjing UniversityXi Chen - Nanjing UniversityHualou Liang - School of Biomedical Engineering, Science, and Health Systems (1997-)
- Publication Details
- RNA biology, v 12(3), pp 276-289
- Publisher
- Taylor & Francis
- Number of pages
- 14
- Grant note
- BK2011013; BK2012014 / Natural Science Foundation of Jiangsu Province 201302018 / Research Special Fund for Public Welfare Industry of Health 2014CB542300 / National Basic Research Program of China (973 Program); National Basic Research Program of China 81101330; 31271378; 81250044 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) NCET-120261 / program for New Century Excellent Talents in University from the Ministry of Education, China
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000352241500006
- Scopus ID
- 2-s2.0-84928265689
- Other Identifier
- 991019320714204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology