Journal article
pH Dependence of Ferricytochrome c Conformational Transitions during Binding to Cardiolipin Membranes: Evidence for Histidine as the Distal Ligand at Neutral pH
The journal of physical chemistry letters, v 8(9), pp 1993-1998
04 May 2017
PMID: 28418677
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The conformational changes of ferricytochrome c upon binding to cardiolipin-containing small unilamellar vesicles were studied at slightly acidic pH using fluorescence, visible circular dichroism, UV-visible absorption, and resonance Raman spectroscopy. The obtained spectroscopic response data suggest a mode of interaction, which is clearly distinct from the binding process observed at neutral pH. Evidence of a reversible and electrostatic binding mechanism under these conditions is provided through binding inhibition in the presence of 150 mM NaCl. Moreover, UV-visible absorption and resonance Raman spectra reveal that the conformational ensemble of membrane bound cytochrome c is dominated by a mixture of conformers with pentacoordinated and hexacoordinated high-spin heme irons, which contrast with the dominance of low-spin species at neutral pH. While our results confirm the L-site binding proposed by Kawai et al., they point to the protonation of a histidine ligand (H33) as conformational trigger.
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Details
- Title
- pH Dependence of Ferricytochrome c Conformational Transitions during Binding to Cardiolipin Membranes: Evidence for Histidine as the Distal Ligand at Neutral pH
- Creators
- Bridget Milorey - Drexel UniversityDmitry Malyshka - Drexel UniversityReinhard Schweitzer-Stenner - Drexel University
- Publication Details
- The journal of physical chemistry letters, v 8(9), pp 1993-1998
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 6
- Grant note
- Department of Chemistry at Drexel University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Chemistry
- Web of Science ID
- WOS:000400885600013
- Scopus ID
- 2-s2.0-85018773486
- Other Identifier
- 991019168088504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Atomic, Molecular & Chemical